Nanoemulsions of cancer chemopreventive agent benzyl isothiocyanate display enhanced solubility, dissolution, and permeability

Hussaini Syed Sha Qhattal; Shu Wang; Tri Salihima; Sanjay K. Srivastava; Xinli Liu

doi:10.1021/jf202612b

Nanoemulsions of cancer chemopreventive agent benzyl isothiocyanate display enhanced solubility, dissolution, and permeability

Hussaini Syed Sha Qhattal, Shu Wang, Tri Salihima, Sanjay K. Srivastava, Xinli Liu

Nutritional Sciences

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

Benzyl isothiocyanate (BITC), a compound found in cruciferous vegetables, is an effective chemopreventive agent. The objective of this study was to develop nanoemulsion formulations for the oral delivery of BITC. Optimized oil-in-water BITC nanoemulsions were prepared by a spontaneous self-nanoemulsification method and a homogenization-sonication method. Both nanoemulsions entrapped high amounts of BITC (15-17 mg/mL), with low polydispersity and good colloidal stability. The BITC nanoemulsions showed enhanced solubility and dissolution compared to pure BITC. These formulations markedly increased the apical to basolateral transport of BITC in Caco-2 cell monolayers. The apparent permeability values were 3.6 × 10 ^-6 cm/s for pure BITC and (1.1-1.3) × 10 ^-5 cm/s for BITC nanoemulsions. The nanoemulsions were easily taken up by human cancer cells A549 and SKOV-3 and inhibited tumor growth in vitro. This work shows for the first time that BITC can be formulated into nanoemulsions and may show promise in enhancing absorption and bioavailability.

Original language	English
Pages (from-to)	12396-12404
Number of pages	9
Journal	Journal of Agricultural and Food Chemistry
Volume	59
Issue number	23
DOIs	https://doi.org/10.1021/jf202612b
State	Published - Dec 14 2011

Keywords

Benzyl isothiocyanate
Caco-2 permeability
chemoprevention
dissolution
nanoemulsion
self-nanoemulsifying drug delivery system

Access to Document

10.1021/jf202612b

Cite this

@article{d99953b510984131ab9e945d266c9e3e,

title = "Nanoemulsions of cancer chemopreventive agent benzyl isothiocyanate display enhanced solubility, dissolution, and permeability",

abstract = "Benzyl isothiocyanate (BITC), a compound found in cruciferous vegetables, is an effective chemopreventive agent. The objective of this study was to develop nanoemulsion formulations for the oral delivery of BITC. Optimized oil-in-water BITC nanoemulsions were prepared by a spontaneous self-nanoemulsification method and a homogenization-sonication method. Both nanoemulsions entrapped high amounts of BITC (15-17 mg/mL), with low polydispersity and good colloidal stability. The BITC nanoemulsions showed enhanced solubility and dissolution compared to pure BITC. These formulations markedly increased the apical to basolateral transport of BITC in Caco-2 cell monolayers. The apparent permeability values were 3.6 × 10 -6 cm/s for pure BITC and (1.1-1.3) × 10 -5 cm/s for BITC nanoemulsions. The nanoemulsions were easily taken up by human cancer cells A549 and SKOV-3 and inhibited tumor growth in vitro. This work shows for the first time that BITC can be formulated into nanoemulsions and may show promise in enhancing absorption and bioavailability.",

keywords = "Benzyl isothiocyanate, Caco-2 permeability, chemoprevention, dissolution, nanoemulsion, self-nanoemulsifying drug delivery system",

author = "Qhattal, {Hussaini Syed Sha} and Shu Wang and Tri Salihima and Srivastava, {Sanjay K.} and Xinli Liu",

year = "2011",

month = dec,

day = "14",

doi = "10.1021/jf202612b",

language = "English",

volume = "59",

pages = "12396--12404",

journal = "Journal of Agricultural and Food Chemistry",

issn = "0021-8561",

publisher = "Journal of agricultural and food chemistry",

number = "23",

}

TY - JOUR

T1 - Nanoemulsions of cancer chemopreventive agent benzyl isothiocyanate display enhanced solubility, dissolution, and permeability

AU - Qhattal, Hussaini Syed Sha

AU - Wang, Shu

AU - Salihima, Tri

AU - Srivastava, Sanjay K.

AU - Liu, Xinli

PY - 2011/12/14

Y1 - 2011/12/14

N2 - Benzyl isothiocyanate (BITC), a compound found in cruciferous vegetables, is an effective chemopreventive agent. The objective of this study was to develop nanoemulsion formulations for the oral delivery of BITC. Optimized oil-in-water BITC nanoemulsions were prepared by a spontaneous self-nanoemulsification method and a homogenization-sonication method. Both nanoemulsions entrapped high amounts of BITC (15-17 mg/mL), with low polydispersity and good colloidal stability. The BITC nanoemulsions showed enhanced solubility and dissolution compared to pure BITC. These formulations markedly increased the apical to basolateral transport of BITC in Caco-2 cell monolayers. The apparent permeability values were 3.6 × 10 -6 cm/s for pure BITC and (1.1-1.3) × 10 -5 cm/s for BITC nanoemulsions. The nanoemulsions were easily taken up by human cancer cells A549 and SKOV-3 and inhibited tumor growth in vitro. This work shows for the first time that BITC can be formulated into nanoemulsions and may show promise in enhancing absorption and bioavailability.

AB - Benzyl isothiocyanate (BITC), a compound found in cruciferous vegetables, is an effective chemopreventive agent. The objective of this study was to develop nanoemulsion formulations for the oral delivery of BITC. Optimized oil-in-water BITC nanoemulsions were prepared by a spontaneous self-nanoemulsification method and a homogenization-sonication method. Both nanoemulsions entrapped high amounts of BITC (15-17 mg/mL), with low polydispersity and good colloidal stability. The BITC nanoemulsions showed enhanced solubility and dissolution compared to pure BITC. These formulations markedly increased the apical to basolateral transport of BITC in Caco-2 cell monolayers. The apparent permeability values were 3.6 × 10 -6 cm/s for pure BITC and (1.1-1.3) × 10 -5 cm/s for BITC nanoemulsions. The nanoemulsions were easily taken up by human cancer cells A549 and SKOV-3 and inhibited tumor growth in vitro. This work shows for the first time that BITC can be formulated into nanoemulsions and may show promise in enhancing absorption and bioavailability.

KW - Benzyl isothiocyanate

KW - Caco-2 permeability

KW - chemoprevention

KW - dissolution

KW - nanoemulsion

KW - self-nanoemulsifying drug delivery system

UR - http://www.scopus.com/inward/record.url?scp=83055165760&partnerID=8YFLogxK

U2 - 10.1021/jf202612b

DO - 10.1021/jf202612b

M3 - Article

C2 - 22011260

AN - SCOPUS:83055165760

SN - 0021-8561

VL - 59

SP - 12396

EP - 12404

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 23

ER -

Nanoemulsions of cancer chemopreventive agent benzyl isothiocyanate display enhanced solubility, dissolution, and permeability

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this