TY - JOUR
T1 - N-linked oligosaccharides of vomeromodulin, a putative pheromone transporter in rat
AU - Mechref, Yehia
AU - Ma, Weidong
AU - Hao, Gang
AU - Novotny, Milos V.
N1 - Funding Information:
This work was supported by Grant DC 02418 from the National Institute of Deafness and Communicative Disorders, U.S. Department of Health and Human Services and by Grant GM24349 from the National Institute of General Medical Sciences, U.S. Department of Health and Human Services.
PY - 1999/2/16
Y1 - 1999/2/16
N2 - Vomeromodulin, a putative pheromone transporter of the rat vomeronasal organ, was isolated by lectin chromatography, purified, and subjected to a mass spectrometric (MS) system of glycan structural determination. Through a combination of exoglycosidase treatments and measurements by matrix-assisted laser desorption/ionization MS, the N-glycans of vomeromodulin were identified as mainly sialylated and fucosylated biantennary structures. The microheterogeneity of N-glycan structures was also due to the presence of galactose residues with different types of linkages.
AB - Vomeromodulin, a putative pheromone transporter of the rat vomeronasal organ, was isolated by lectin chromatography, purified, and subjected to a mass spectrometric (MS) system of glycan structural determination. Through a combination of exoglycosidase treatments and measurements by matrix-assisted laser desorption/ionization MS, the N-glycans of vomeromodulin were identified as mainly sialylated and fucosylated biantennary structures. The microheterogeneity of N-glycan structures was also due to the presence of galactose residues with different types of linkages.
UR - http://www.scopus.com/inward/record.url?scp=0033573890&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1999.0176
DO - 10.1006/bbrc.1999.0176
M3 - Article
C2 - 10049729
AN - SCOPUS:0033573890
VL - 255
SP - 451
EP - 455
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -