N-linked oligosaccharides of vomeromodulin, a putative pheromone transporter in rat

Yehia Mechref; Weidong Ma; Gang Hao; Milos V. Novotny

doi:10.1006/bbrc.1999.0176

N-linked oligosaccharides of vomeromodulin, a putative pheromone transporter in rat

Yehia Mechref, Weidong Ma, Gang Hao, Milos V. Novotny

Chemistry and Biochemistry

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Vomeromodulin, a putative pheromone transporter of the rat vomeronasal organ, was isolated by lectin chromatography, purified, and subjected to a mass spectrometric (MS) system of glycan structural determination. Through a combination of exoglycosidase treatments and measurements by matrix-assisted laser desorption/ionization MS, the N-glycans of vomeromodulin were identified as mainly sialylated and fucosylated biantennary structures. The microheterogeneity of N-glycan structures was also due to the presence of galactose residues with different types of linkages.

Original language	English
Pages (from-to)	451-455
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	255
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.1999.0176
State	Published - Feb 16 1999

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abstract = "Vomeromodulin, a putative pheromone transporter of the rat vomeronasal organ, was isolated by lectin chromatography, purified, and subjected to a mass spectrometric (MS) system of glycan structural determination. Through a combination of exoglycosidase treatments and measurements by matrix-assisted laser desorption/ionization MS, the N-glycans of vomeromodulin were identified as mainly sialylated and fucosylated biantennary structures. The microheterogeneity of N-glycan structures was also due to the presence of galactose residues with different types of linkages.",

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AU - Ma, Weidong

AU - Hao, Gang

AU - Novotny, Milos V.

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