N-glycan profiling by microchip electrophoresis to differentiate disease states related to esophageal adenocarcinoma

Indranil Mitra, Zexi Zhuang, Yuening Zhang, Chuan Yih Yu, Zane T. Hammoud, Haixu Tang, Yehia Mechref, Stephen C. Jacobson

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

We report analysis of N-glycans derived from disease-free individuals and patients with Barrett's esophagus, high-grade dysplasia, and esophageal adenocarcinoma by microchip electrophoresis with laser-induced fluorescence detection. Serum samples in 10 μL aliquots are enzymatically treated to cleave the N-glycans that are subsequently reacted with 8-aminopyrene-1,3,6- trisulfonic acid to add charge and a fluorescent label. Separations at 1250 V/cm and over 22 cm yielded efficiencies up to 700?000 plates for the N-glycans and analysis times under 100 s. Principal component analysis (PCA) and analysis of variance (ANOVA) tests of the peak areas and migration times are used to evaluate N-glycan profiles from native and desialylated samples and determine differences among the four sample groups. With microchip electrophoresis, we are able to distinguish the three patient groups from each other and from disease-free individuals.

Original languageEnglish
Pages (from-to)3621-3627
Number of pages7
JournalAnalytical Chemistry
Volume84
Issue number8
DOIs
StatePublished - Apr 17 2012

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