TY - JOUR
T1 - Multitargeted Flavonoid Inhibition of the Pathogenic Bacterium Staphylococcus aureus: A Proteomic Characterization
T2 - A Proteomic Characterization
AU - Elmasri, Wael
AU - Zhu, Rui
AU - Peng, Wenjing
AU - Al-Hariri, Moustafa
AU - Kobeissy, Firas
AU - Tran, Phat
AU - Hamood, Abdul N.
AU - Hegazy, Mohamed F.
AU - Paré, Paul W.
AU - Mechref, Yehia
N1 - Funding Information:
This research was supported in part by the Robert Welch Foundation (D-1478), NSF equipment grant CHE-1048553, and the NSF CRIF program.
Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/7/7
Y1 - 2017/7/7
N2 - © 2017 American Chemical Society. Growth inhibition of the pathogen Staphylococcus aureus with currently available antibiotics is problematic in part due to bacterial biofilm protection. Although recently characterized natural products, including 3′,4′,5-trihydroxy-6,7-dimethoxy-flavone [1], 3′,4′,5,6,7-pentahydroxy-flavone [2] , and 5-hydroxy-4′,7-dimethoxy-flavone [3], exhibit both antibiotic and biofilm inhibitory activities, the mode of action of such hydroxylated flavonoids with respect to S. aureus inhibition is yet to be characterized. Enzymatic digestion and high-resolution MS analysis of differentially expressed proteins from S. aureus with and without exposure to antibiotic flavonoids (1-3) allowed for the characterization of global protein alterations induced by metabolite treatment. A total of 56, 92, and 110 proteins were differentially expressed with bacterial exposure to 1, 2, or 3, respectively. The connectivity of the identified proteins was characterized using a sear
AB - © 2017 American Chemical Society. Growth inhibition of the pathogen Staphylococcus aureus with currently available antibiotics is problematic in part due to bacterial biofilm protection. Although recently characterized natural products, including 3′,4′,5-trihydroxy-6,7-dimethoxy-flavone [1], 3′,4′,5,6,7-pentahydroxy-flavone [2] , and 5-hydroxy-4′,7-dimethoxy-flavone [3], exhibit both antibiotic and biofilm inhibitory activities, the mode of action of such hydroxylated flavonoids with respect to S. aureus inhibition is yet to be characterized. Enzymatic digestion and high-resolution MS analysis of differentially expressed proteins from S. aureus with and without exposure to antibiotic flavonoids (1-3) allowed for the characterization of global protein alterations induced by metabolite treatment. A total of 56, 92, and 110 proteins were differentially expressed with bacterial exposure to 1, 2, or 3, respectively. The connectivity of the identified proteins was characterized using a sear
KW - antibacterial flavonoids
KW - LC-MS/MS proteomics
KW - multitargeted bacterial inhibition
KW - Staphylococcus aureus
UR - http://www.scopus.com/inward/record.url?scp=85023198580&partnerID=8YFLogxK
U2 - 10.1021/acs.jproteome.7b00137
DO - 10.1021/acs.jproteome.7b00137
M3 - Article
C2 - 28541047
VL - 16
SP - 2579
EP - 2586
JO - Journal of Proteome Research
JF - Journal of Proteome Research
IS - 7
ER -