Multitargeted Flavonoid Inhibition of the Pathogenic Bacterium Staphylococcus aureus: A Proteomic Characterization

Wael Elmasri, Rui Zhu, Wenjing Peng, Moustafa Al-Hariri, Firas Kobeissy, Phat Tran, Abdul N. Hamood, Mohamed F. Hegazy, Paul W. Paré, Yehia Mechref

Research output: Contribution to journalArticlepeer-review

Abstract

© 2017 American Chemical Society. Growth inhibition of the pathogen Staphylococcus aureus with currently available antibiotics is problematic in part due to bacterial biofilm protection. Although recently characterized natural products, including 3′,4′,5-trihydroxy-6,7-dimethoxy-flavone [1], 3′,4′,5,6,7-pentahydroxy-flavone [2] , and 5-hydroxy-4′,7-dimethoxy-flavone [3], exhibit both antibiotic and biofilm inhibitory activities, the mode of action of such hydroxylated flavonoids with respect to S. aureus inhibition is yet to be characterized. Enzymatic digestion and high-resolution MS analysis of differentially expressed proteins from S. aureus with and without exposure to antibiotic flavonoids (1-3) allowed for the characterization of global protein alterations induced by metabolite treatment. A total of 56, 92, and 110 proteins were differentially expressed with bacterial exposure to 1, 2, or 3, respectively. The connectivity of the identified proteins was characterized using a sear
Original languageEnglish
Pages (from-to)2579-2586
JournalJournal of Proteome Research
StatePublished - Jul 7 2017

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