TY - JOUR
T1 - Multiple antibiotic resistance genes distribution in ten large-scale membrane bioreactors for municipal wastewater treatment
AU - Sun, Yanmei
AU - Shen, Yue xiao
AU - Liang, Peng
AU - Zhou, Jizhong
AU - Yang, Yunfeng
AU - Huang, Xia
N1 - Funding Information:
This work was supported by the Key Program of the National Natural Science Foundation of China (No. 51238004 ), and the Program for Outstanding PhD thesis of Beijing (No. 20131000305).
Publisher Copyright:
© 2016 Elsevier Ltd
PY - 2016/12/1
Y1 - 2016/12/1
N2 - Wastewater treatment plants are thought to be potential reservoirs of antibiotic resistance genes. In this study, GeoChip was used for analyzing multiple antibiotic resistance genes, including four multidrug efflux system gene groups and three β-lactamase genes in ten large-scale membrane bioreactors (MBRs) for municipal wastewater treatment. Results revealed that the diversity of antibiotic genes varied a lot among MBRs, but about 40% common antibiotic resistance genes were existent. The average signal intensity of each antibiotic resistance group was similar among MBRs, nevertheless the total abundance of each group varied remarkably and the dominant resistance gene groups were different in individual MBR. The antibiotic resistance genes majorly derived from Proteobacteria and Actinobacteria. Further study indicated that TN, TP and COD of influent, temperature and conductivity of mixed liquor were significant (P < 0.05) correlated to the multiple antibiotic resistance genes distribution in MBRs.
AB - Wastewater treatment plants are thought to be potential reservoirs of antibiotic resistance genes. In this study, GeoChip was used for analyzing multiple antibiotic resistance genes, including four multidrug efflux system gene groups and three β-lactamase genes in ten large-scale membrane bioreactors (MBRs) for municipal wastewater treatment. Results revealed that the diversity of antibiotic genes varied a lot among MBRs, but about 40% common antibiotic resistance genes were existent. The average signal intensity of each antibiotic resistance group was similar among MBRs, nevertheless the total abundance of each group varied remarkably and the dominant resistance gene groups were different in individual MBR. The antibiotic resistance genes majorly derived from Proteobacteria and Actinobacteria. Further study indicated that TN, TP and COD of influent, temperature and conductivity of mixed liquor were significant (P < 0.05) correlated to the multiple antibiotic resistance genes distribution in MBRs.
KW - GeoChip
KW - Membrane bioreactors
KW - Multiple antibiotic resistance genes
KW - Municipal wastewater
UR - http://www.scopus.com/inward/record.url?scp=84989879384&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2016.09.117
DO - 10.1016/j.biortech.2016.09.117
M3 - Article
C2 - 27716561
AN - SCOPUS:84989879384
SN - 0960-8524
VL - 222
SP - 100
EP - 106
JO - Bioresource Technology
JF - Bioresource Technology
ER -