Different analytical methods have been developed to effectively and accurately determine monosaccharide composition of glycoproteins and glycolipids in biological and biomedical samples. These methods included high-performance anion-exchange chromatography with pulse amperometric detection (HPAEC-PAD), high-performance liquid chromatography with fluorescence detection (HPLC-FL), and capillary electrophoresis with laser-induced fluorescence detection (CE-LIF). This chapter reviews the development of these methods, and evaluates their potential application in the diagnosis or prognosis of diseases.
|Title of host publication||Capillary Electrophoresis of Carbohydrates|
|Subtitle of host publication||From Monosaccharides to Complex Polysaccharides|
|Publisher||Humana Press Inc.|
|Number of pages||31|
|State||Published - 2011|
- Capillary electrophoresis
- Laser-induced fluorescence