Abstract
Different analytical methods have been developed to effectively and accurately determine monosaccharide composition of glycoproteins and glycolipids in biological and biomedical samples. These methods included high-performance anion-exchange chromatography with pulse amperometric detection (HPAEC-PAD), high-performance liquid chromatography with fluorescence detection (HPLC-FL), and capillary electrophoresis with laser-induced fluorescence detection (CE-LIF). This chapter reviews the development of these methods, and evaluates their potential application in the diagnosis or prognosis of diseases.
| Original language | English |
|---|---|
| Title of host publication | Capillary Electrophoresis of Carbohydrates |
| Subtitle of host publication | From Monosaccharides to Complex Polysaccharides |
| Publisher | Humana Press Inc. |
| Pages | 237-267 |
| Number of pages | 31 |
| ISBN (Electronic) | 9781607618751 |
| ISBN (Print) | 9781607618744 |
| DOIs | |
| State | Published - 2011 |
Keywords
- Capillary electrophoresis
- Diagnosis
- Glycolipids
- Glycoproteins
- HPAEC-PAD
- HPLC-FL
- HPLC-MS
- Laser-induced fluorescence
- Monosaccharides
- Oligosaccharides
- Prognosis