Molecular detection of Oxyspirura larvae in arthropod intermediate hosts

Sadia Almas, Anna G. Gibson, Steven M. Presley

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


To determine potential intermediate hosts of Oxyspirura petrowi, a common nematode eyeworm of wild gallinaceous birds, various arthropod species including red harvester ants, beetles, wood cockroaches, crickets, grasshoppers, katydids, and desert termites were screened for the presence of O. petrowi using specific polymerase chain reaction (PCR) primers targeting the internal transcribed spacer 2 region (ITS2) of the eyeworm ribosomal deoxyribonucleic acid (rDNA). This is the first study to investigate the intermediate hosts of O. petrowi utilizing molecular techniques. We determined 38% (13/34) of the cockroaches, 27% (3/11) of the crickets, and 23% (68/289) of the grasshoppers which were positive for O. petrowi. Identifying potential intermediate hosts of O. petrowi is essential to better understanding the epizoology of the eyeworm’s transmission mechanics and to controlling infections in wild gallinaceous birds.

Original languageEnglish
Pages (from-to)819-823
Number of pages5
JournalParasitology Research
Issue number3
StatePublished - Mar 1 2018


  • Bobwhite quail
  • Cockroach
  • Galliformes
  • Grasshopper
  • Intermediate hosts
  • Oxyspirura petrowi
  • Parasitic nematodes


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