Modulation and study of photoblinking behavior in dye doped silver-silica core-shell nanoparticles for localization super-resolution microscopy

Chumki Chakraborty, S. Thompson, Veronica J. Lyons, Craig Snoeyink, Dimitri Pappas

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Blinking of fluorescent nanoparticles is a compelling phenomenon with widely debated mechanisms. The ability to inhibit or control blinking is important for applications in the field of optical, semiconductor and fluorescent imaging. Self-blinking nanomaterials are also attractive labels for localization-based super-resolution microscopy. In this work, we have synthesized silver core silica nanoparticles (Ag@SiO2) doped with Rhodamine 110 and studied the parameters that affect blinking. We found that under nitrogen rich conditions the nanoparticles shifted towards higher duty cycles. Also, it was found that hydrated nanoparticles showed a less drastic response to nitrogen rich conditions as compared to dried nanoparticles, indicating that surrounding matrix played a role in the response of nanoparticles to molecular oxygen. Further, the blinking is not a multi-body phenomena, super-resolution localization combined with intensity histogram analysis confirmed that single particles are emitting.

Original languageEnglish
Article number455704
JournalNanotechnology
Volume30
Issue number45
DOIs
StatePublished - Aug 22 2019

Fingerprint

Dive into the research topics of 'Modulation and study of photoblinking behavior in dye doped silver-silica core-shell nanoparticles for localization super-resolution microscopy'. Together they form a unique fingerprint.

Cite this