TY - JOUR
T1 - Modeling studies of cell response to ultrashort, high-intensity electric fields - Implications for intracellular manipulation
AU - Joshi, Ravindra P.
AU - Hu, Qin
AU - Schoenbach, Karl H.
N1 - Funding Information:
Manuscript received August 9, 2003; revised October 3, 2003. This work was sponsored in part by the Air Force Office of Scientific Research (#F49620-01-1-0506) on Bio-Inspired Concepts and an AFOSR-MURI Grant (#F49620-02-1-0320) on Subcellular Responses to Narrowband and Wideband Radio Frequency Radiation.
PY - 2004/8
Y1 - 2004/8
N2 - The dynamics of electroporation in biological cells subjected to nanosecond, high-intensity pulses are studied based on a coupled scheme involving both the current continuity and Smoluchowski equations. A new distributed network model, that includes dynamic conductivities of cell membranes and substructures, is introduced for evaluations of transmembrane potential. It is shown that subcellular structures could be affected through nanosecond pulses, and that, despite the high field intensity, the processes remain nonthermal. As an example of selectivity, differences in cell responses between normal and malignant (Farage) tonsillar B-cells are compared and discussed. It is shown that ultrashort, high-intensity electric pulses could damage cancer cells. Finally, the model predicts that it is possible to target the inner mitochondrial membrane (i.e., selectivity at the organelle level), in keeping with recent experimental observations.
AB - The dynamics of electroporation in biological cells subjected to nanosecond, high-intensity pulses are studied based on a coupled scheme involving both the current continuity and Smoluchowski equations. A new distributed network model, that includes dynamic conductivities of cell membranes and substructures, is introduced for evaluations of transmembrane potential. It is shown that subcellular structures could be affected through nanosecond pulses, and that, despite the high field intensity, the processes remain nonthermal. As an example of selectivity, differences in cell responses between normal and malignant (Farage) tonsillar B-cells are compared and discussed. It is shown that ultrashort, high-intensity electric pulses could damage cancer cells. Finally, the model predicts that it is possible to target the inner mitochondrial membrane (i.e., selectivity at the organelle level), in keeping with recent experimental observations.
UR - http://www.scopus.com/inward/record.url?scp=7244242189&partnerID=8YFLogxK
U2 - 10.1109/TPS.2004.830971
DO - 10.1109/TPS.2004.830971
M3 - Article
AN - SCOPUS:7244242189
SN - 0093-3813
VL - 32
SP - 1677
EP - 1686
JO - IEEE Transactions on Plasma Science
JF - IEEE Transactions on Plasma Science
IS - 4 II
ER -