Miniaturized separation techniques in glycomic investigations

Yehia Mechref; Milos V. Novotny

doi:10.1016/j.jchromb.2006.04.049

Miniaturized separation techniques in glycomic investigations

Yehia Mechref, Milos V. Novotny

Research output: Contribution to journal › Review article › peer-review

67 Scopus citations

Abstract

High-sensitivity glycomic analyses are becoming of a great interest in modern biomedical and clinical research, as well as in the development of recombinant protein products. The evolution of separation techniques for glycomic analysis at high sensitivity is highlighted in this review. These methodologies include capillary liquid chromatography, capillary electrophoresis (CE) and capillary electrochromatography (CEC). The potential of such methodologies in glycomic analysis is demonstrated for model glycoproteins as well as total glycomes derived from biological samples.

Original language	English
Pages (from-to)	65-78
Number of pages	14
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	841
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jchromb.2006.04.049
State	Published - Sep 1 2006

Keywords

Capillary electrochromatography
Capillary electrophoresis
Capillary liquid chromatography
Glycomics
Graphitized carbon packings
Hydrophilic nano-LC
Oligosaccharides
Permethylation

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10.1016/j.jchromb.2006.04.049

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title = "Miniaturized separation techniques in glycomic investigations",

abstract = "High-sensitivity glycomic analyses are becoming of a great interest in modern biomedical and clinical research, as well as in the development of recombinant protein products. The evolution of separation techniques for glycomic analysis at high sensitivity is highlighted in this review. These methodologies include capillary liquid chromatography, capillary electrophoresis (CE) and capillary electrochromatography (CEC). The potential of such methodologies in glycomic analysis is demonstrated for model glycoproteins as well as total glycomes derived from biological samples.",

keywords = "Capillary electrochromatography, Capillary electrophoresis, Capillary liquid chromatography, Glycomics, Graphitized carbon packings, Hydrophilic nano-LC, Oligosaccharides, Permethylation",

author = "Yehia Mechref and Novotny, {Milos V.}",

note = "Funding Information: The authors acknowledge financial supports through by Grant No. (GM24349) from the National Institute of General Medical Sciences, U.S. Department of Health and Human Services, a grant from the Indiana 21st Century Research and Technology Fund, and the National Center for Glycomics and Glycoproteomics, funded by by NIH-National Center for Research Resources (NCRR).",

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doi = "10.1016/j.jchromb.2006.04.049",

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AU - Novotny, Milos V.

N1 - Funding Information: The authors acknowledge financial supports through by Grant No. (GM24349) from the National Institute of General Medical Sciences, U.S. Department of Health and Human Services, a grant from the Indiana 21st Century Research and Technology Fund, and the National Center for Glycomics and Glycoproteomics, funded by by NIH-National Center for Research Resources (NCRR).

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N2 - High-sensitivity glycomic analyses are becoming of a great interest in modern biomedical and clinical research, as well as in the development of recombinant protein products. The evolution of separation techniques for glycomic analysis at high sensitivity is highlighted in this review. These methodologies include capillary liquid chromatography, capillary electrophoresis (CE) and capillary electrochromatography (CEC). The potential of such methodologies in glycomic analysis is demonstrated for model glycoproteins as well as total glycomes derived from biological samples.

AB - High-sensitivity glycomic analyses are becoming of a great interest in modern biomedical and clinical research, as well as in the development of recombinant protein products. The evolution of separation techniques for glycomic analysis at high sensitivity is highlighted in this review. These methodologies include capillary liquid chromatography, capillary electrophoresis (CE) and capillary electrochromatography (CEC). The potential of such methodologies in glycomic analysis is demonstrated for model glycoproteins as well as total glycomes derived from biological samples.

KW - Capillary electrochromatography

KW - Capillary electrophoresis

KW - Capillary liquid chromatography

KW - Glycomics

KW - Graphitized carbon packings

KW - Hydrophilic nano-LC

KW - Oligosaccharides

KW - Permethylation

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DO - 10.1016/j.jchromb.2006.04.049

M3 - Review article

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VL - 841

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JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

SN - 1570-0232

IS - 1-2

ER -

Miniaturized separation techniques in glycomic investigations

Abstract

Keywords

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