Miniaturized separation techniques in glycomic investigations

Yehia Mechref, Milos V. Novotny

Research output: Contribution to journalReview article

64 Scopus citations

Abstract

High-sensitivity glycomic analyses are becoming of a great interest in modern biomedical and clinical research, as well as in the development of recombinant protein products. The evolution of separation techniques for glycomic analysis at high sensitivity is highlighted in this review. These methodologies include capillary liquid chromatography, capillary electrophoresis (CE) and capillary electrochromatography (CEC). The potential of such methodologies in glycomic analysis is demonstrated for model glycoproteins as well as total glycomes derived from biological samples.

Original languageEnglish
Pages (from-to)65-78
Number of pages14
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume841
Issue number1-2
DOIs
StatePublished - Sep 1 2006

Keywords

  • Capillary electrochromatography
  • Capillary electrophoresis
  • Capillary liquid chromatography
  • Glycomics
  • Graphitized carbon packings
  • Hydrophilic nano-LC
  • Oligosaccharides
  • Permethylation

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