TY - JOUR
T1 - microRNA-directed phasing during trans-acting siRNA biogenesis in plants
AU - Allen, Edwards
AU - Xie, Zhixin
AU - Gustafson, Adam M.
AU - Carrington, James C.
N1 - Funding Information:
This work was supported by grants from the National Science Foundation (MCB-0209836), National Institutes of Health (AI43288), and USDA (2005-35319-15280). We thank Anne-Marie Girard for assistance with microarray experiments and Scott Givan, Chris Sullivan, and Daniel Smith for assistance and advice with computational resources. We thank Scott Poethig and Manabu Yoshikawa for generously providing seed and RNA for rdr6-11 and sgs3-11 mutants. We also thank lab members for advice and discussion.
PY - 2005/4/22
Y1 - 2005/4/22
N2 - Plants and animals use small RNAs (microRNAs [miRNAs] and siRNAs) as guides for posttranscriptional and epigenetic regulation. In plants, miRNAs and trans-acting (ta) siRNAs form through distinct biogenesis pathways, although they both interact with target transcripts and guide cleavage. An integrated approach to identify targets of Arabidopsis thaliana miRNAs and ta-siRNAs revealed several new classes of small RNA-regulated genes, including conventional genes such as Argonaute2 and an E2-ubiquitin conjugating enzyme. Surprisingly, five ta-siRNA-generating transcripts were identified as targets of miR173 or miR390. Rather than functioning as negative regulators, miR173- and miR390-guided cleavage was shown to set the 21-nucleotide phase for ta-siRNA precursor processing. These data support a model in which miRNA-guided formation of a 5′ or 3′ terminus within pre-ta-siRNA transcripts, followed by RDR6-dependent formation of dsRNA and Dicer-like processing, yields phased ta-siRNAs that negatively regulate other genes.
AB - Plants and animals use small RNAs (microRNAs [miRNAs] and siRNAs) as guides for posttranscriptional and epigenetic regulation. In plants, miRNAs and trans-acting (ta) siRNAs form through distinct biogenesis pathways, although they both interact with target transcripts and guide cleavage. An integrated approach to identify targets of Arabidopsis thaliana miRNAs and ta-siRNAs revealed several new classes of small RNA-regulated genes, including conventional genes such as Argonaute2 and an E2-ubiquitin conjugating enzyme. Surprisingly, five ta-siRNA-generating transcripts were identified as targets of miR173 or miR390. Rather than functioning as negative regulators, miR173- and miR390-guided cleavage was shown to set the 21-nucleotide phase for ta-siRNA precursor processing. These data support a model in which miRNA-guided formation of a 5′ or 3′ terminus within pre-ta-siRNA transcripts, followed by RDR6-dependent formation of dsRNA and Dicer-like processing, yields phased ta-siRNAs that negatively regulate other genes.
UR - http://www.scopus.com/inward/record.url?scp=17444430392&partnerID=8YFLogxK
U2 - 10.1016/j.cell.2005.04.004
DO - 10.1016/j.cell.2005.04.004
M3 - Article
C2 - 15851028
AN - SCOPUS:17444430392
SN - 0092-8674
VL - 121
SP - 207
EP - 221
JO - Cell
JF - Cell
IS - 2
ER -