Microbial Profile Evaluation of Beef Steaks From Different Packaging and Retail Lighting Display Conditions

Keelyn E. Hanlon, Joshua C. McCann, Mark F. Miller, Mindy Brashears, Colton L. Smith, J Brooks, Jerrad Legako

Research output: Contribution to journalArticlepeer-review

Abstract

<jats:p>To date, meat microbiology research has relied on culture-dependent methods. Amplicon sequencing technology provides a deeper look into the microbial community. This study set out to evaluate the bacterial community of fresh beef longissimus lumborum steaks exposed to retail packaging and display conditions. Four packaging treatments were assigned after fabrication 7 d postmortem: high-oxygen modified atmosphere packaging, overwrapped packages within a carbon monoxide tri-gas flushed motherbag, vacuum rollstock pouches, and traditional overwrap. After a 14-d dark storage, carbon monoxide motherbag overwrapped packages were removed from the motherbag, and packages were distributed to a retail lighting condition for 72 h of retail display: fluorescent, light emitting diode, or darkness. Aerobic plate count and psychrotrophic bacteria were enumerated, in addition to 16S ribosomal RNA sequencing of DNA for microbial profile investigation. Sampling occurred at fabrication (7 d), en
Original languageEnglish
JournalMeat and Muscle Biology
DOIs
StatePublished - Jun 2021

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