Microbial Profile Evaluation of Beef Steaks From Different Packaging and Retail Lighting Display Conditions

Keelyn E. Hanlon; Joshua C. McCann; Mark F. Miller; Mindy Brashears; Colton L. Smith; J Brooks; Jerrad Legako

doi:10.22175/mmb.11711

Microbial Profile Evaluation of Beef Steaks From Different Packaging and Retail Lighting Display Conditions

Keelyn E. Hanlon, Joshua C. McCann, Mark F. Miller, Mindy Brashears, Colton L. Smith, J Brooks, Jerrad Legako

Research output: Contribution to journal › Article › peer-review

Abstract

To date, meat microbiology research has relied on culture-dependent methods. Amplicon sequencing technology provides a deeper look into the microbial community. This study set out to evaluate the bacterial community of fresh beef longissimus lumborum steaks exposed to retail packaging and display conditions. Four packaging treatments were assigned after fabrication 7 d postmortem: high-oxygen modified atmosphere packaging, overwrapped packages within a carbon monoxide tri-gas flushed motherbag, vacuum rollstock pouches, and traditional overwrap. After a 14-d dark storage, carbon monoxide motherbag overwrapped packages were removed from the motherbag, and packages were distributed to a retail lighting condition for 72 h of retail display: fluorescent, light emitting diode, or darkness. Aerobic plate count and psychrotrophic bacteria were enumerated, in addition to 16S ribosomal RNA sequencing of DNA for microbial profile investigation. Sampling occurred at fabrication (7 d), en

Original language	English
Journal	Meat and Muscle Biology
DOIs	https://doi.org/10.22175/mmb.11711
State	Published - Jun 2021

Access to Document

10.22175/mmb.11711

Cite this

@article{ae7f5c229b2c4d088246de9aa6f05537,

title = "Microbial Profile Evaluation of Beef Steaks From Different Packaging and Retail Lighting Display Conditions",

abstract = "To date, meat microbiology research has relied on culture-dependent methods. Amplicon sequencing technology provides a deeper look into the microbial community. This study set out to evaluate the bacterial community of fresh beef longissimus lumborum steaks exposed to retail packaging and display conditions. Four packaging treatments were assigned after fabrication 7 d postmortem: high-oxygen modified atmosphere packaging, overwrapped packages within a carbon monoxide tri-gas flushed motherbag, vacuum rollstock pouches, and traditional overwrap. After a 14-d dark storage, carbon monoxide motherbag overwrapped packages were removed from the motherbag, and packages were distributed to a retail lighting condition for 72 h of retail display: fluorescent, light emitting diode, or darkness. Aerobic plate count and psychrotrophic bacteria were enumerated, in addition to 16S ribosomal RNA sequencing of DNA for microbial profile investigation. Sampling occurred at fabrication (7 d), en",

author = "Hanlon, {Keelyn E.} and McCann, {Joshua C.} and Miller, {Mark F.} and Mindy Brashears and Smith, {Colton L.} and J Brooks and Jerrad Legako",

year = "2021",

month = jun,

doi = "10.22175/mmb.11711",

language = "English",

journal = "Meat and Muscle Biology",

publisher = "American Meat Science Association",

}

TY - JOUR

T1 - Microbial Profile Evaluation of Beef Steaks From Different Packaging and Retail Lighting Display Conditions

AU - Hanlon, Keelyn E.

AU - McCann, Joshua C.

AU - Miller, Mark F.

AU - Brashears, Mindy

AU - Smith, Colton L.

AU - Brooks, J

AU - Legako, Jerrad

PY - 2021/6

Y1 - 2021/6

N2 - To date, meat microbiology research has relied on culture-dependent methods. Amplicon sequencing technology provides a deeper look into the microbial community. This study set out to evaluate the bacterial community of fresh beef longissimus lumborum steaks exposed to retail packaging and display conditions. Four packaging treatments were assigned after fabrication 7 d postmortem: high-oxygen modified atmosphere packaging, overwrapped packages within a carbon monoxide tri-gas flushed motherbag, vacuum rollstock pouches, and traditional overwrap. After a 14-d dark storage, carbon monoxide motherbag overwrapped packages were removed from the motherbag, and packages were distributed to a retail lighting condition for 72 h of retail display: fluorescent, light emitting diode, or darkness. Aerobic plate count and psychrotrophic bacteria were enumerated, in addition to 16S ribosomal RNA sequencing of DNA for microbial profile investigation. Sampling occurred at fabrication (7 d), en

AB - To date, meat microbiology research has relied on culture-dependent methods. Amplicon sequencing technology provides a deeper look into the microbial community. This study set out to evaluate the bacterial community of fresh beef longissimus lumborum steaks exposed to retail packaging and display conditions. Four packaging treatments were assigned after fabrication 7 d postmortem: high-oxygen modified atmosphere packaging, overwrapped packages within a carbon monoxide tri-gas flushed motherbag, vacuum rollstock pouches, and traditional overwrap. After a 14-d dark storage, carbon monoxide motherbag overwrapped packages were removed from the motherbag, and packages were distributed to a retail lighting condition for 72 h of retail display: fluorescent, light emitting diode, or darkness. Aerobic plate count and psychrotrophic bacteria were enumerated, in addition to 16S ribosomal RNA sequencing of DNA for microbial profile investigation. Sampling occurred at fabrication (7 d), en

U2 - 10.22175/mmb.11711

DO - 10.22175/mmb.11711

M3 - Article

JO - Meat and Muscle Biology

JF - Meat and Muscle Biology

ER -

Microbial Profile Evaluation of Beef Steaks From Different Packaging and Retail Lighting Display Conditions

Abstract

Access to Document

Fingerprint

Cite this