Melengestrol acetate alters muscle cell proliferation in heifers and steers

E. K. Sissom, C. D. Reinhardt, B. J. Johnson

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


In vitro experiments were performed to investigate the effects of melengestrol acetate (MGA) or progesterone (P4) on bovine muscle satellite cells and C2C12 myoblasts. Addition of MGA at physiological and supraphysiological concentrations resulted in a dose-dependent decrease (P < 0.05) in DNA synthesis as measured by 3H]-thymidine incorporation (TI). Similarly, P4 addition (0.01 nM) reduced (P < 0.05) TI. Addition of MGA (10 nM) increased (P < 0.05) IGF-I mRNA abundance but did not affect myogenin mRNA. Progesterone addition (10 nM) increased myogenin mRNA abundance (P < 0.05). In C2C12 cultures, P4 addition resulted in a dose-dependent decrease in TI. The antiprogestin RU486, in combination with MGA or P4, also resulted in reduced (P < 0.05) TI. Treatment with RU486 alone had a negative effect (P < 0.05) on TI that was similar to the progestins. Treatment of C2C12 myoblasts with MGA (100 nM) resulted in an increase (P < 0.05) in myogenin mRNA. These studies suggest that progestins may reduce satellite cell proliferation, ultimately affecting carcass composition.

Original languageEnglish
Pages (from-to)2950-2958
Number of pages9
JournalJournal of animal science
Issue number11
StatePublished - Nov 2006


  • Bovine
  • Carcass
  • Insulin-like growth factor-I
  • Melengestrol acetate
  • Progesterone
  • Satellite cell


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