Measuring Complexation by Single Molecule Fluorescence Anisotropy

Sean Burrows, Dimitrios Pappas

Research output: Contribution to journalArticlepeer-review


The complexation of a fluorescent probe by a target protein was observed by single molecule fluorescence anisotropy. Free and bound states, heterogeneities, and rare binding events can all be observed by this approach. Fluorophore-conjugated biotin was used to bind to neutravidin as a proof of concept case. Molecular interactions were observed that could not be elucidated with conventional (ensemble) measurements.
Original languageEnglish
Pages (from-to)870-873
JournalThe Analyst
StatePublished - 2008


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