Abstract
The complexation of a fluorescent probe by a target protein was observed by single molecule fluorescence anisotropy. Free and bound states, heterogeneities, and rare binding events can all be observed by this approach. Fluorophore-conjugated biotin was used to bind to neutravidin as a proof of concept case. Molecular interactions were observed that could not be elucidated with conventional (ensemble) measurements.
| Original language | English |
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| Pages (from-to) | 870-873 |
| Journal | The Analyst |
| State | Published - 2008 |