Measuring Complexation by Single Molecule Fluorescence Anisotropy

The complexation of a fluorescent probe by a target protein was observed by single molecule fluorescence anisotropy. Free and bound states, heterogeneities, and rare binding events can all be observed by this approach. Fluorophore-conjugated biotin was used to bind to neutravidin as a proof of concept case. Molecular interactions were observed that could not be elucidated with conventional (ensemble) measurements.