Measuring complexation by single-molecule fluorescence anisotropy

Sean M. Burrows, Dimitri Pappas

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


The complexation of a fluorescent probe by a target protein was observed by single-molecule fluorescence anisotropy. Free and bound states, heterogeneities, and rare binding events can all be observed by this approach. Fluorophore-conjugated biotin was used to bind to NeutrAvidin as a proof-of-concept case. Molecular interactions were observed that could not be elucidated with conventional (ensemble) measurements.

Original languageEnglish
Pages (from-to)870-873
Number of pages4
Issue number7
StatePublished - 2008


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