Matrix-assisted laser desorption/ionization mass spectrometry of acidic glycoconjugates facilitated by the use of spermine as a co-matrix

Y. Mechref, M. V. Novotny

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

Negative-ion matrix-assisted laser desorption/ionization mass spectra of sialylated glycoconjugates were acquired employing 2,5-dihydroxybenzoic acid (DHB) in conjunction with spermine as a co-matrix. The addition of spermine to DHB permitted an improved crystal formation as well as a higher analyte solubility. Moreover, DHB/spermine appears to minimize alkali adduct formation, thus allowing the sample analysis without desalting. The combined matrix permitted the analysis of complex sialylated and sialylated/fucosylated structures down to the femtomole range. The ability to use such a matrix also facilitates determination of the sialic acid linkages (in combination with a specific enzyme cleavage). The matrix also appears suitable for studies on gangliosides.

Original languageEnglish
Pages (from-to)1293-1302
Number of pages10
JournalJournal of the American Society for Mass Spectrometry
Volume9
Issue number12
DOIs
StatePublished - 1998

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