Matrix-assisted laser desorption/ionization mass spectrometry compatible β-elimination of O-linked oligosaccharides

Yunping Huang, Tomonori Konse, Yehia Mechref, Milos V. Novotny

Research output: Contribution to journalArticle

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Abstract

A new β-elimination procedure has been introduced to cleave O-linked oligosaccharides from low-to sub-microgram amounts of glycoproteins prior to analysis by mass spectrometry. Borane-ammonia complex in aqueous ammonia is used as a cleaving solution alternative to the sodium borohydride/ sodium hydroxide medium conventionally used in β-elimination. The procedure results in minimum sample purification, leading to minimal sample loss and consequently an overall enhancement in sensitivity. It was applied successfully in the analysis of bovine fetuin and submaxillary mucin, as well as to a complex bile-salt-stimulated lipase glycoprotein isolated from human milk.

Original languageEnglish
Pages (from-to)1199-1204
Number of pages6
JournalRapid Communications in Mass Spectrometry
Volume16
Issue number12
DOIs
StatePublished - Jan 1 2002

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