Mass Spectrometric Mapping and Sequencing of N-Linked Oligosaccharides Derived from Submicrogram Amounts of Glycoproteins

Yehia Mechref, Milos V. Novotny

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119 Scopus citations

Abstract

Very small quantities of glycoproteins were directiy processed on a MALDI sampling plate prior to their mass spectrometric investigations. The on-plate digestion with N-glycanase released effectively the corresponding oligosaccharides in very short times, irrespective of their molecular mass. The following treatment with an array of exoglycosidase enzymes enables sequencing and a linkage-form determination in analysis times that are considerably shorter than achieved previously: the entire structural determination on a glycoprotein can be completed in one day, with a minimum substrate consumption. Ribonuclease B, bovine fetuin, human α1-acid glycoprotein, and the diamine oxidase (from porcine kidney) have been used to illustrate different aspects of the on-plate sample treatment/MALDI mass spectrometry.

Original languageEnglish
Pages (from-to)455-463
Number of pages9
JournalAnalytical Chemistry
Volume70
Issue number3
DOIs
StatePublished - Feb 1 1998

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