TY - JOUR
T1 - Marker-assisted introgression and stacking of major QTLs controlling grain number (Grain number 1a (Gn1a)) and number of primary branching (Wealthy Farmer’s Panicle (WFP)) to NERICA cultivars.
AU - Reyes, Vincent P.
AU - Shim, Rosalyn
AU - Mendioro, Merlyn S.
AU - Manuel, Ma Carmina C.
AU - Lapis, Ruby S.
AU - Shim, Junghyun
AU - Sunohara, Hidehiko
AU - Nishiuchi, Shunsaku
AU - Kikuta, Mayumi
AU - Makihara, Daigo
AU - Jena, Kshirod
AU - Ashikari, Motoyuki
AU - Doi, Kazuyuki
PY - 2021/4/22
Y1 - 2021/4/22
N2 - The era of the green revolution has significantly improved rice yield productivity. However,with the growing population and decreasing arable land, rice scientists must find new ways toimprove rice productivity. Although hundreds of rice yield-related QTLs were already mapped andsome of them were cloned, only a few were utilized for actual systematic introgression breedingprograms. In this study, the major yield QTLs Grain Number 1a (Gn1a) and Wealthy Farmer’s Panicle(WFP) were introgressed and stacked in selected NERICA cultivars by marker-assisted backcrossbreeding (MABB). The DNA markers RM3360, RM3452, and RM5493 were used for foregroundselection. At BC3F4 and BC3F5 generation, a combination of marker-assisted selection and phenotypicevaluation were carried out to select lines with target alleles and traits. Further, genotyping-bysequencing(GBS) was conducted to validate the introgression and determine the recurrent parentgenome recovery (RPGR) of the selected lines. The Gn1a and/
AB - The era of the green revolution has significantly improved rice yield productivity. However,with the growing population and decreasing arable land, rice scientists must find new ways toimprove rice productivity. Although hundreds of rice yield-related QTLs were already mapped andsome of them were cloned, only a few were utilized for actual systematic introgression breedingprograms. In this study, the major yield QTLs Grain Number 1a (Gn1a) and Wealthy Farmer’s Panicle(WFP) were introgressed and stacked in selected NERICA cultivars by marker-assisted backcrossbreeding (MABB). The DNA markers RM3360, RM3452, and RM5493 were used for foregroundselection. At BC3F4 and BC3F5 generation, a combination of marker-assisted selection and phenotypicevaluation were carried out to select lines with target alleles and traits. Further, genotyping-bysequencing(GBS) was conducted to validate the introgression and determine the recurrent parentgenome recovery (RPGR) of the selected lines. The Gn1a and/
U2 - 10.3390/plants10050844
DO - 10.3390/plants10050844
M3 - Article
SP - 844
JO - Plants
JF - Plants
ER -