Marker-assisted introgression and stacking of major QTLs controlling grain number (Grain number 1a (Gn1a)) and number of primary branching (Wealthy Farmer’s Panicle (WFP)) to NERICA cultivars.

Vincent P. Reyes, Rosalyn Shim, Merlyn S. Mendioro, Ma Carmina C. Manuel, Ruby S. Lapis, Junghyun Shim, Hidehiko Sunohara, Shunsaku Nishiuchi, Mayumi Kikuta, Daigo Makihara, Kshirod Jena, Motoyuki Ashikari, Kazuyuki Doi

Research output: Contribution to journalArticlepeer-review

Abstract

The era of the green revolution has significantly improved rice yield productivity. However,with the growing population and decreasing arable land, rice scientists must find new ways toimprove rice productivity. Although hundreds of rice yield-related QTLs were already mapped andsome of them were cloned, only a few were utilized for actual systematic introgression breedingprograms. In this study, the major yield QTLs Grain Number 1a (Gn1a) and Wealthy Farmer’s Panicle(WFP) were introgressed and stacked in selected NERICA cultivars by marker-assisted backcrossbreeding (MABB). The DNA markers RM3360, RM3452, and RM5493 were used for foregroundselection. At BC3F4 and BC3F5 generation, a combination of marker-assisted selection and phenotypicevaluation were carried out to select lines with target alleles and traits. Further, genotyping-bysequencing(GBS) was conducted to validate the introgression and determine the recurrent parentgenome recovery (RPGR) of the selected lines. The
Original languageEnglish
Pages (from-to)844
JournalPlants
DOIs
StatePublished - Apr 22 2021

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