@article{2d3eecb42a9e48f5b3263a9f0245fc25,
title = "Marker-assisted introgression and stacking of major qtls controlling grain number (Gn1a) and number of primary branching (wfp) to nerica cultivars",
abstract = "The era of the green revolution has significantly improved rice yield productivity. However, with the growing population and decreasing arable land, rice scientists must find new ways to improve rice productivity. Although hundreds of rice yield-related QTLs were already mapped and some of them were cloned, only a few were utilized for actual systematic introgression breeding programs. In this study, the major yield QTLs Grain Number 1a (Gn1a) and Wealthy Farmer{\textquoteright}s Panicle (WFP) were introgressed and stacked in selected NERICA cultivars by marker-assisted backcross breeding (MABB). The DNA markers RM3360, RM3452, and RM5493 were used for foreground selection. At BC3 F4 and BC3 F5 generation, a combination of marker-assisted selection and phenotypic evaluation were carried out to select lines with target alleles and traits. Further, genotyping-by-sequencing (GBS) was conducted to validate the introgression and determine the recurrent parent genome recovery (RPGR) of the selected lines. The Gn1a and/or WFP introgression lines showed significantly higher numbers of spikelets per panicle and primary branching compared to the recurrent parents. In addition, lines with Gn1a and/or WFP alleles were comparatively similar to the recurrent parents (RP) in most yield-related traits. This study demonstrates the success of utilizing yield QTLs and marker-assisted selection to develop and improve rice cultivars.",
keywords = "Crop improvement, Genotyping-by-sequencing, Gn1a, Marker-assisted backcrossing, Molecular breeding, NERICA, Rice yield, WFP",
author = "Reyes, {Vincent P.} and Angeles-Shim, {Rosalyn B.} and Mendioro, {Merlyn S.} and Manuel, {Ma Carmina C.} and Lapis, {Ruby S.} and Junghyun Shim and Hidehiko Sunohara and Shunsaku Nishiuchi and Mayumi Kikuta and Daigo Makihara and Jena, {Kshirod K.} and Motoyuki Ashikari and Kazuyuki Doi",
note = "Funding Information: Funding: V.P.R. is grateful to MEXT, Japan, for the scholarship. This research was funded by Japan International Cooperation Agency (JICA) as part of the Wonder Rice Initiative for Food Security and Health (WISH) Project, the Canon Foundation, the SATREPS project entitled {\textquoteleft}Improvement in Productivity and Yield Stability of Rice under Kenya{\textquoteright}s Biotic and Abiotic Stress Conditions through Tailor-made Breeding and Development of Cultivation Methods{\textquoteright}, the RIKEN Cluster for Science, Technology and Innovation Hub (RCSTI), and JSPS KAKENHI Grant Number JP18H02187. Funding Information: V.P.R. is grateful to MEXT, Japan, for the scholarship. This research was funded by Japan International Cooperation Agency (JICA) as part of the Wonder Rice Initiative for Food Security and Health (WISH) Project, the Canon Foundation, the SATREPS project entitled ?Improvement in Productivity and Yield Stability of Rice under Kenya?s Biotic and Abiotic Stress Conditions through Tailor-made Breeding and Development of Cultivation Methods?, the RIKEN Cluster for Science, Technology and Innovation Hub (RCSTI), and JSPS KAKENHI Grant Number JP18H02187. The authors would like to thank Angelito A. Aragon and Maricar Maullion for providing technical support in the maintenance of experimental materials. V.P.R. would like to thank DOST-ASTHRDP for the support during his MSc study. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2021",
doi = "10.3390/plants10050844",
language = "English",
volume = "10",
journal = "Plants",
issn = "2223-7747",
number = "5",
}