Mapping site-specific protein N-glycosylations through liquid chromatography/mass spectrometry and targeted tandem mass spectrometry

Yin Wu, Yehia Mechref, Iveta Klouckova, Anoop Mayampurath, Milos V. Novotny, Haixu Tang

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Glycosylation is one of the most common posttranslational modifications (PTMs) of proteins, the characterization of which is commonly achieved through proteomic protocol, involving trypsin digestion followed by liquid chromatography/tandem mass spectrometry (LC/MS/MS). However, it is often not possible to characterize all glycopeptides in a complex sample because of the high complexity of glycoproteomic samples, and the relative lower abundances of glycopeptides in comparison to the unmodified peptides. We presen the reatargeted MS/MS analysis approach, which utilizes a previously developed computational tool, GlyPID, to guide multiple experiments, thus permitting a complete characterization of all N-glycosylation sites of glycoproteins present in a complex sample. We have tested our approach using model glycoproteins analyzed by high-resolution LTQ-FT MS. The results demonstrate a potential use of our method for a high-throughput characterization of complex mixtures of glycosylated proteins.

Original languageEnglish
Pages (from-to)965-972
Number of pages8
JournalRapid Communications in Mass Spectrometry
Volume24
Issue number7
DOIs
StatePublished - Apr 15 2010

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