LPS-induced immune system stimulation alters urinary volatiles and behavior in growing pigs

John McGlone; Sankarganesh Devaraj; Edgar Aviles-Rosa; Anoosh Rakhshandeh

doi:10.1007/978-3-030-17616-7_5

LPS-induced immune system stimulation alters urinary volatiles and behavior in growing pigs

John McGlone, Sankarganesh Devaraj, Edgar Aviles-Rosa, Anoosh Rakhshandeh

Animal and Food Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Volatile compounds from body fluids and secretions may indicate the health status of individuals to other conspecifics. We sought to identify urinary volatiles that might act as biomarkers indicative of the health status of growing pigs. Immune system stimulation (ISS) was induced in pigs (N = 5) by intramuscular (IM) injection of bacterial lipopolysaccharide (LPS; 25 µg/kg BW). Urine samples were collected before (baseline) and after LPS treatment. The urinary headspace volatile compounds were identified using Solid-phase microextraction (SPME) coupled with GC-MS. Pig eye temperatures was measured using an infrared imaging technique, and behaviours (i.e. standing, feeding, drinking, moving, lying, sitting and active) were video-recorded and quantified 24 h before and after ISS. Data were analysed using mixed model ANOVA examining periods before and after LPS administration with pigs serving as their own control. Pig eye/body temperature increased post-ISS (at 6 h, P = 0.03) a

Original language	English
Title of host publication	LPS-induced immune system stimulation alters urinary volatiles and behavior in growing pigs
Publisher	Springer Nature
Pages	60-70
Volume	14
DOIs	https://doi.org/10.1007/978-3-030-17616-7_5
State	Published - Jul 20 2019

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abstract = "Volatile compounds from body fluids and secretions may indicate the health status of individuals to other conspecifics. We sought to identify urinary volatiles that might act as biomarkers indicative of the health status of growing pigs. Immune system stimulation (ISS) was induced in pigs (N = 5) by intramuscular (IM) injection of bacterial lipopolysaccharide (LPS; 25 µg/kg BW). Urine samples were collected before (baseline) and after LPS treatment. The urinary headspace volatile compounds were identified using Solid-phase microextraction (SPME) coupled with GC-MS. Pig eye temperatures was measured using an infrared imaging technique, and behaviours (i.e. standing, feeding, drinking, moving, lying, sitting and active) were video-recorded and quantified 24 h before and after ISS. Data were analysed using mixed model ANOVA examining periods before and after LPS administration with pigs serving as their own control. Pig eye/body temperature increased post-ISS (at 6 h, P = 0.03) a",

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LPS-induced immune system stimulation alters urinary volatiles and behavior in growing pigs. / McGlone, John; Devaraj, Sankarganesh; Aviles-Rosa, Edgar; Rakhshandeh, Anoosh.

LPS-induced immune system stimulation alters urinary volatiles and behavior in growing pigs. Vol. 14 Springer Nature, 2019. p. 60-70.

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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