Low Temperature Plasma Probe Mass Spectrometry for Analytes Separated on Thin-Layer Chromatography Plates: Direct vs Laser Assisted Desorption

Xiaoxia Gong, Dong Zhang, Inah B. Embile, Yue She, Songyue Shi, Gerardo Gamez Goytia

Research output: Contribution to journalArticlepeer-review

Abstract

Thin-layer chromatography (TLC) is a widespread technique because it allows fast, simple and inexpensive analyte separations. In addition, direct analysis of the compounds separated on TLC plates via mass spectrometry (MS) has shown to provide high sensitivity and selectivity while avoiding time-consuming sample extraction protocols. Here, direct desorption low temperature plasma mass spectrometry (LTP-MS) as well as diode laser assisted desorption (LD) LTP-MS are studied for direct spatially-resolved<br>analysis of compounds from TLC plates. Qualitative and quantitative characterization of amino acids, pharmaceuticals and structural isomers were performed. The nature of the TLC plate stationary phase was found to have a significant influence, together with the analyte’s characteristics, on the desorption efficiency. Tandem MS is shown to greatly improve the limits of detection (LODs). Direct desorption LTP-MS, without external thermal assisted desorption, demonstrates its best perfor
Original languageEnglish
JournalJournal of the American Society for Mass Spectrometry
DOIs
StatePublished - Jul 2020

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