TY - JOUR
T1 - Low Temperature Plasma Probe Mass Spectrometry for Analytes Separated on Thin-Layer Chromatography Plates: Direct vs Laser Assisted Desorption
AU - Gong, Xiaoxia
AU - Zhang, Dong
AU - Embile, Inah B.
AU - She, Yue
AU - Shi, Songyue
AU - Gamez Goytia, Gerardo
PY - 2020/7
Y1 - 2020/7
N2 - Thin-layer chromatography (TLC) is a widespread technique because it allows fast, simple and inexpensive analyte separations. In addition, direct analysis of the compounds separated on TLC plates via mass spectrometry (MS) has shown to provide high sensitivity and selectivity while avoiding time-consuming sample extraction protocols. Here, direct desorption low temperature plasma mass spectrometry (LTP-MS) as well as diode laser assisted desorption (LD) LTP-MS are studied for direct spatially-resolvedanalysis of compounds from TLC plates. Qualitative and quantitative characterization of amino acids, pharmaceuticals and structural isomers were performed. The nature of the TLC plate stationary phase was found to have a significant influence, together with the analyte’s characteristics, on the desorption efficiency. Tandem MS is shown to greatly improve the limits of detection (LODs). Direct desorption LTP-MS, without external thermal assisted desorption, demonstrates its best perfor
AB - Thin-layer chromatography (TLC) is a widespread technique because it allows fast, simple and inexpensive analyte separations. In addition, direct analysis of the compounds separated on TLC plates via mass spectrometry (MS) has shown to provide high sensitivity and selectivity while avoiding time-consuming sample extraction protocols. Here, direct desorption low temperature plasma mass spectrometry (LTP-MS) as well as diode laser assisted desorption (LD) LTP-MS are studied for direct spatially-resolvedanalysis of compounds from TLC plates. Qualitative and quantitative characterization of amino acids, pharmaceuticals and structural isomers were performed. The nature of the TLC plate stationary phase was found to have a significant influence, together with the analyte’s characteristics, on the desorption efficiency. Tandem MS is shown to greatly improve the limits of detection (LODs). Direct desorption LTP-MS, without external thermal assisted desorption, demonstrates its best perfor
U2 - 10.1021/jasms.0c00246
DO - 10.1021/jasms.0c00246
M3 - Article
JO - Journal of the American Society for Mass Spectrometry
JF - Journal of the American Society for Mass Spectrometry
ER -