Abstract
lin-Benzo-adenine nucleotides can act not only as probes for fluorescence studies but also as structural active site probes for enzymes. To understand the basic properties of lin-benzo-ATP and-ADP, protolysis and Mg2+ and Ca2+, binding are investigated between pH 6.2 and pH 8.5 by spectrophotometric and spectrofluorometric titrations. Based on a reaction model, a set of equilibrium constants is determined which is consistent with all available experimental results. The p K values of the Mg2+ and Ca2+ complex of lin-benzo-ATP in the chosen medium are 4.6 and 4.1, respectively, and those for the corresponding diphosphate are 3.1 and 2.8, respectively. Fluorescence and absorption spectra are reported.
Original language | English |
---|---|
Pages (from-to) | 247-250 |
Number of pages | 4 |
Journal | Journal of Fluorescence |
Volume | 4 |
Issue number | 3 |
DOIs | |
State | Published - Sep 1994 |
Keywords
- Mg and Ca binding
- dissociation constants
- fluorescence spectra
- lin-Benzo-ATP
- lin-benzo-ADP