lin-Benzo-ATP and-ADP: Versatile fluorescent probes for spectroscopic and biochemical studies

E. Grell; E. Lewitzki; C. Bremer; S. Kramer-Schmitt; J. Weber; A. E. Senior

doi:10.1007/BF01878458

lin-Benzo-ATP and-ADP: Versatile fluorescent probes for spectroscopic and biochemical studies

E. Grell, E. Lewitzki, C. Bremer, S. Kramer-Schmitt, J. Weber, A. E. Senior

Chemistry and Biochemistry

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

lin-Benzo-adenine nucleotides can act not only as probes for fluorescence studies but also as structural active site probes for enzymes. To understand the basic properties of lin-benzo-ATP and-ADP, protolysis and Mg²⁺ and Ca²⁺, binding are investigated between pH 6.2 and pH 8.5 by spectrophotometric and spectrofluorometric titrations. Based on a reaction model, a set of equilibrium constants is determined which is consistent with all available experimental results. The p K values of the Mg²⁺ and Ca²⁺ complex of lin-benzo-ATP in the chosen medium are 4.6 and 4.1, respectively, and those for the corresponding diphosphate are 3.1 and 2.8, respectively. Fluorescence and absorption spectra are reported.

Original language	English
Pages (from-to)	247-250
Number of pages	4
Journal	Journal of Fluorescence
Volume	4
Issue number	3
DOIs	https://doi.org/10.1007/BF01878458
State	Published - Sep 1994

Keywords

Mg and Ca binding
dissociation constants
fluorescence spectra
lin-Benzo-ATP
lin-benzo-ADP

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10.1007/BF01878458

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title = "lin-Benzo-ATP and-ADP: Versatile fluorescent probes for spectroscopic and biochemical studies",

abstract = "lin-Benzo-adenine nucleotides can act not only as probes for fluorescence studies but also as structural active site probes for enzymes. To understand the basic properties of lin-benzo-ATP and-ADP, protolysis and Mg2+ and Ca2+, binding are investigated between pH 6.2 and pH 8.5 by spectrophotometric and spectrofluorometric titrations. Based on a reaction model, a set of equilibrium constants is determined which is consistent with all available experimental results. The p K values of the Mg2+ and Ca2+ complex of lin-benzo-ATP in the chosen medium are 4.6 and 4.1, respectively, and those for the corresponding diphosphate are 3.1 and 2.8, respectively. Fluorescence and absorption spectra are reported.",

keywords = "Mg and Ca binding, dissociation constants, fluorescence spectra, lin-Benzo-ATP, lin-benzo-ADP",

author = "E. Grell and E. Lewitzki and C. Bremer and S. Kramer-Schmitt and J. Weber and Senior, {A. E.}",

year = "1994",

month = sep,

doi = "10.1007/BF01878458",

language = "English",

volume = "4",

pages = "247--250",

journal = "Journal of Fluorescence",

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T1 - lin-Benzo-ATP and-ADP

T2 - Versatile fluorescent probes for spectroscopic and biochemical studies

AU - Grell, E.

AU - Lewitzki, E.

AU - Bremer, C.

AU - Kramer-Schmitt, S.

AU - Weber, J.

AU - Senior, A. E.

PY - 1994/9

Y1 - 1994/9

N2 - lin-Benzo-adenine nucleotides can act not only as probes for fluorescence studies but also as structural active site probes for enzymes. To understand the basic properties of lin-benzo-ATP and-ADP, protolysis and Mg2+ and Ca2+, binding are investigated between pH 6.2 and pH 8.5 by spectrophotometric and spectrofluorometric titrations. Based on a reaction model, a set of equilibrium constants is determined which is consistent with all available experimental results. The p K values of the Mg2+ and Ca2+ complex of lin-benzo-ATP in the chosen medium are 4.6 and 4.1, respectively, and those for the corresponding diphosphate are 3.1 and 2.8, respectively. Fluorescence and absorption spectra are reported.

AB - lin-Benzo-adenine nucleotides can act not only as probes for fluorescence studies but also as structural active site probes for enzymes. To understand the basic properties of lin-benzo-ATP and-ADP, protolysis and Mg2+ and Ca2+, binding are investigated between pH 6.2 and pH 8.5 by spectrophotometric and spectrofluorometric titrations. Based on a reaction model, a set of equilibrium constants is determined which is consistent with all available experimental results. The p K values of the Mg2+ and Ca2+ complex of lin-benzo-ATP in the chosen medium are 4.6 and 4.1, respectively, and those for the corresponding diphosphate are 3.1 and 2.8, respectively. Fluorescence and absorption spectra are reported.

KW - Mg and Ca binding

KW - dissociation constants

KW - fluorescence spectra

KW - lin-Benzo-ATP

KW - lin-benzo-ADP

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