lin-Benzo-ATP and-ADP: Versatile fluorescent probes for spectroscopic and biochemical studies

E. Grell, E. Lewitzki, C. Bremer, S. Kramer-Schmitt, J. Weber, A. E. Senior

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

lin-Benzo-adenine nucleotides can act not only as probes for fluorescence studies but also as structural active site probes for enzymes. To understand the basic properties of lin-benzo-ATP and-ADP, protolysis and Mg2+ and Ca2+, binding are investigated between pH 6.2 and pH 8.5 by spectrophotometric and spectrofluorometric titrations. Based on a reaction model, a set of equilibrium constants is determined which is consistent with all available experimental results. The p K values of the Mg2+ and Ca2+ complex of lin-benzo-ATP in the chosen medium are 4.6 and 4.1, respectively, and those for the corresponding diphosphate are 3.1 and 2.8, respectively. Fluorescence and absorption spectra are reported.

Original languageEnglish
Pages (from-to)247-250
Number of pages4
JournalJournal of Fluorescence
Volume4
Issue number3
DOIs
StatePublished - Sep 1994

Keywords

  • Mg and Ca binding
  • dissociation constants
  • fluorescence spectra
  • lin-Benzo-ATP
  • lin-benzo-ADP

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