Light-inducible and chloroplast-associated expression of a chimaeric gene introduced into Nicotiana tabacum using a Ti plasmid vector

L. Herrera-Estrella; G. Van Den Broeck; R. Maenhaut; M. Van Montagu; J. Schell; M. Timko; A. Cashmore

doi:10.1038/310115a0

Light-inducible and chloroplast-associated expression of a chimaeric gene introduced into Nicotiana tabacum using a Ti plasmid vector

L. Herrera-Estrella, G. Van Den Broeck, R. Maenhaut, M. Van Montagu, J. Schell, M. Timko, A. Cashmore

Plant & Soil Science

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Abstract

A chimaeric gene, consisting of the 5′-flanking region of a member of the Pisum sativum gene family encoding ribulose 1,5-bisphosphate carboxylase linked to the coding region of a bacterial chloramphenicol acetyltransferase gene, has been introduced into the genome of the plant Nicotiana tabacum using a Ti plasmid of Agrobacterium tumefaciens. The expression of the chimaeric gene is light-inducible in chloroplast-containing transformed tissue.

Original language	English
Pages (from-to)	115-120
Number of pages	6
Journal	Nature
Volume	310
Issue number	5973
DOIs	https://doi.org/10.1038/310115a0
State	Published - 1984

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title = "Light-inducible and chloroplast-associated expression of a chimaeric gene introduced into Nicotiana tabacum using a Ti plasmid vector",

abstract = "A chimaeric gene, consisting of the 5′-flanking region of a member of the Pisum sativum gene family encoding ribulose 1,5-bisphosphate carboxylase linked to the coding region of a bacterial chloramphenicol acetyltransferase gene, has been introduced into the genome of the plant Nicotiana tabacum using a Ti plasmid of Agrobacterium tumefaciens. The expression of the chimaeric gene is light-inducible in chloroplast-containing transformed tissue.",

author = "L. Herrera-Estrella and {Van Den Broeck}, G. and R. Maenhaut and {Van Montagu}, M. and J. Schell and M. Timko and A. Cashmore",

year = "1984",

doi = "10.1038/310115a0",

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T1 - Light-inducible and chloroplast-associated expression of a chimaeric gene introduced into Nicotiana tabacum using a Ti plasmid vector

AU - Herrera-Estrella, L.

AU - Van Den Broeck, G.

AU - Maenhaut, R.

AU - Van Montagu, M.

AU - Schell, J.

AU - Timko, M.

AU - Cashmore, A.

PY - 1984

Y1 - 1984

N2 - A chimaeric gene, consisting of the 5′-flanking region of a member of the Pisum sativum gene family encoding ribulose 1,5-bisphosphate carboxylase linked to the coding region of a bacterial chloramphenicol acetyltransferase gene, has been introduced into the genome of the plant Nicotiana tabacum using a Ti plasmid of Agrobacterium tumefaciens. The expression of the chimaeric gene is light-inducible in chloroplast-containing transformed tissue.

AB - A chimaeric gene, consisting of the 5′-flanking region of a member of the Pisum sativum gene family encoding ribulose 1,5-bisphosphate carboxylase linked to the coding region of a bacterial chloramphenicol acetyltransferase gene, has been introduced into the genome of the plant Nicotiana tabacum using a Ti plasmid of Agrobacterium tumefaciens. The expression of the chimaeric gene is light-inducible in chloroplast-containing transformed tissue.

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DO - 10.1038/310115a0

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EP - 120

JO - Nature

JF - Nature

IS - 5973

ER -

Light-inducible and chloroplast-associated expression of a chimaeric gene introduced into Nicotiana tabacum using a Ti plasmid vector

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