Leukocyte-Reduced Platelet-Rich Plasma Normalizes Matrix Metabolism in Torn Human Rotator Cuff Tendons

J Cross; Brian Cole; K Spatny; Emily Sundman; A Romeo; G Nicholson; B Wagner; Lisa Fortier

Leukocyte-Reduced Platelet-Rich Plasma Normalizes Matrix Metabolism in Torn Human Rotator Cuff Tendons

J Cross, Brian Cole, K Spatny, Emily Sundman, A Romeo, G Nicholson, B Wagner, Lisa Fortier

Veterinary Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Background: The optimal platelet-rich plasma (PRP) for treatment of supraspinatus tendinopathy has not been determined. Purpose: To evaluate the effect of low- versus high-leukocyte concentrated PRP products on catabolic and anabolic mediators of matrix metabolism in diseased rotator cuff tendons. Study design: Controlled laboratory study. Methods: Diseased supraspinatus tendons were treated with PRP made by use of 2 commercial systems: Arthrex Autologous Conditioned Plasma Double Syringe System (L(lo) PRP) and Biomet GPS III Mini Platelet Concentrate System (L(hi) PRP). Tendon explants were placed in 6-well plates and cultured in L(lo) PRP, L(hi) PRP, or control media (Dulbecco's Modified Eagle Medium + 10% fetal bovine serum) for 96 hours. Tendo

Original language	English
Pages (from-to)	2898-2906
Journal	American Journal of Sports Medicine
State	Published - Dec 2015

Cite this

@article{970dffc298954fadb60fd55782f6ba10,

title = "Leukocyte-Reduced Platelet-Rich Plasma Normalizes Matrix Metabolism in Torn Human Rotator Cuff Tendons",

abstract = "Background: The optimal platelet-rich plasma (PRP) for treatment of supraspinatus tendinopathy has not been determined. Purpose: To evaluate the effect of low- versus high-leukocyte concentrated PRP products on catabolic and anabolic mediators of matrix metabolism in diseased rotator cuff tendons. Study design: Controlled laboratory study. Methods: Diseased supraspinatus tendons were treated with PRP made by use of 2 commercial systems: Arthrex Autologous Conditioned Plasma Double Syringe System (L(lo) PRP) and Biomet GPS III Mini Platelet Concentrate System (L(hi) PRP). Tendon explants were placed in 6-well plates and cultured in L(lo) PRP, L(hi) PRP, or control media (Dulbecco's Modified Eagle Medium + 10% fetal bovine serum) for 96 hours. Tendo",

author = "J Cross and Brian Cole and K Spatny and Emily Sundman and A Romeo and G Nicholson and B Wagner and Lisa Fortier",

year = "2015",

month = dec,

language = "English",

pages = "2898--2906",

journal = "American Journal of Sports Medicine",

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TY - JOUR

T1 - Leukocyte-Reduced Platelet-Rich Plasma Normalizes Matrix Metabolism in Torn Human Rotator Cuff Tendons

AU - Cross, J

AU - Cole, Brian

AU - Spatny, K

AU - Sundman, Emily

AU - Romeo, A

AU - Nicholson, G

AU - Wagner, B

AU - Fortier, Lisa

PY - 2015/12

Y1 - 2015/12

N2 - Background: The optimal platelet-rich plasma (PRP) for treatment of supraspinatus tendinopathy has not been determined. Purpose: To evaluate the effect of low- versus high-leukocyte concentrated PRP products on catabolic and anabolic mediators of matrix metabolism in diseased rotator cuff tendons. Study design: Controlled laboratory study. Methods: Diseased supraspinatus tendons were treated with PRP made by use of 2 commercial systems: Arthrex Autologous Conditioned Plasma Double Syringe System (L(lo) PRP) and Biomet GPS III Mini Platelet Concentrate System (L(hi) PRP). Tendon explants were placed in 6-well plates and cultured in L(lo) PRP, L(hi) PRP, or control media (Dulbecco's Modified Eagle Medium + 10% fetal bovine serum) for 96 hours. Tendo

AB - Background: The optimal platelet-rich plasma (PRP) for treatment of supraspinatus tendinopathy has not been determined. Purpose: To evaluate the effect of low- versus high-leukocyte concentrated PRP products on catabolic and anabolic mediators of matrix metabolism in diseased rotator cuff tendons. Study design: Controlled laboratory study. Methods: Diseased supraspinatus tendons were treated with PRP made by use of 2 commercial systems: Arthrex Autologous Conditioned Plasma Double Syringe System (L(lo) PRP) and Biomet GPS III Mini Platelet Concentrate System (L(hi) PRP). Tendon explants were placed in 6-well plates and cultured in L(lo) PRP, L(hi) PRP, or control media (Dulbecco's Modified Eagle Medium + 10% fetal bovine serum) for 96 hours. Tendo

M3 - Article

SP - 2898

EP - 2906

JO - American Journal of Sports Medicine

JF - American Journal of Sports Medicine

ER -

Leukocyte-Reduced Platelet-Rich Plasma Normalizes Matrix Metabolism in Torn Human Rotator Cuff Tendons

Abstract

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