LC-MS/MS in glycomics and glycoproteomics analyses

Mona Goli, Aiying Yu, Byeong Gwan Cho, Sakshi Gautam, Junyao Wang, Cristian D. Gutierrez-Reyes, Peilin Jiang, Wenjing Peng, Yehia Mechref

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


Glycomic and glycoproteomic analyses include the characterization of oligosaccharides (glycans) conjugated to proteins. Glycan characterization is critical to obtain a comprehensive vision of the regulation and functions of glycoproteins of interest. Glycans are created through a complicated nontemplate-driven process by a set of competing enzymes that lengthen the embryonic chain. The complex nature of these structures, stemming from variable compositions and linkages, and the poor ionization efficiency make it challenging to characterize the oligosaccharides. However, there have been significant developments and progressive separation, in which mass spectrometry (MS) approaches have been at the center and have been the main reason for this development. The primary purpose of this chapter is to discuss and evaluate the current LC separation techniques conjugated with MS, with an emphasis on the isomeric resolution of glycans and glycopeptides.

Original languageEnglish
Title of host publicationCarbohydrate Analysis by Modern Liquid Phase Separation Techniques
Number of pages51
ISBN (Electronic)9780128214473
StatePublished - Jan 1 2021


  • Glycomics
  • Glycoproteomics
  • Glycosylation
  • Isomeric separation
  • Mass spectrometry
  • Software tools


Dive into the research topics of 'LC-MS/MS in glycomics and glycoproteomics analyses'. Together they form a unique fingerprint.

Cite this