LC-MS/MS analysis of permethylated N-glycans facilitating isomeric characterization

Shiyue Zhou, Xue Dong, Lucas Veillon, Yifan Huang, Yehia Mechref

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

The biosynthesis of glycans is a template-free process; hence compositionally identical glycans may contain highly heterogeneous structures. Meanwhile, the functions of glycans in biological processes are significantly influenced by the glycan structure. Structural elucidation of glycans is an essential component of glycobiology. Although NMR is considered the most powerful approach for structural glycan studies, it suffers from low sensitivity and requires highly purified glycans. Although mass spectrometry (MS)-based methods have been applied in numerous glycan structure studies, there are challenges in preserving glycan structure during ionization. Permethylation is an efficient derivatization method that improves glycan structural stability. In this report, permethylated glycans are isomerically separated; thus facilitating structural analysis of a mixture of glycans by LC-MS/MS. Separation by porous graphitic carbon liquid chromatography at high temperatures in conjunction with tandem mass spectrometry (PGC-LC-MS/MS) was utilized for unequivocal characterization of glycan isomers. Glycan fucosylation sites were confidently determined by eliminating fucose rearrangement and assignment of diagnostic ions, achieved by permethylation and PGC-LC at high temperatures, respectively. Assigning monosaccharide residues to specific glycan antennae was also achieved. Galactose linkages were also distinguished from each other by CID/HCD tandem MS. This was attainable because of the different bond energies associated with monosaccharide linkages. [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)453-466
Number of pages14
JournalAnalytical and Bioanalytical Chemistry
Volume409
Issue number2
DOIs
StatePublished - Jan 1 2017

Keywords

  • LC-MS/MS
  • N-Glycan isomers
  • Permethylation
  • Porous graphitic carbon column
  • Tandem mass spectrometry

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