Laser-induced photofragmentation of neutral and acidic glycans inside an ion-trap mass spectrometer

Arugadoss Devakumar; Yehia Mechref; Pilsoo Kang; Milos V. Novotny; James P. Reilly

doi:10.1002/rcm.2981

Laser-induced photofragmentation of neutral and acidic glycans inside an ion-trap mass spectrometer

Arugadoss Devakumar, Yehia Mechref, Pilsoo Kang, Milos V. Novotny, James P. Reilly

Chemistry and Biochemistry

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

Permethylated acidic and neutral N-glycans representing different types of glycan structures, such as linear and branched sialylated structures, high-mannose type and fucosylated complex type, were photodissociated with 157 nm vacuum ultraviolet light in a linear ion trap. Cross-ring fragments corresponding to high-energy fragmentation pathways were observed in abundance for all studied structures. Some product ions appear diagnostic for a linkage of sialic acid residues and the glycan antenna to which these residues are attached. A conclusive assignment of the fucosylation site of the studied glycan structure has been facilitated through measurement of cross-ring fragmentation resulting from photodissociation.

Original language	English
Pages (from-to)	1452-1460
Number of pages	9
Journal	Rapid Communications in Mass Spectrometry
Volume	21
Issue number	8
DOIs	https://doi.org/10.1002/rcm.2981
State	Published - 2007

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AU - Devakumar, Arugadoss

AU - Mechref, Yehia

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AU - Novotny, Milos V.

AU - Reilly, James P.

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