Abstract
Permethylated acidic and neutral N-glycans representing different types of glycan structures, such as linear and branched sialylated structures, high-mannose type and fucosylated complex type, were photodissociated with 157 nm vacuum ultraviolet light in a linear ion trap. Cross-ring fragments corresponding to high-energy fragmentation pathways were observed in abundance for all studied structures. Some product ions appear diagnostic for a linkage of sialic acid residues and the glycan antenna to which these residues are attached. A conclusive assignment of the fucosylation site of the studied glycan structure has been facilitated through measurement of cross-ring fragmentation resulting from photodissociation.
Original language | English |
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Pages (from-to) | 1452-1460 |
Number of pages | 9 |
Journal | Rapid Communications in Mass Spectrometry |
Volume | 21 |
Issue number | 8 |
DOIs | |
State | Published - 2007 |