Laser-induced photofragmentation of neutral and acidic glycans inside an ion-trap mass spectrometer

Arugadoss Devakumar, Yehia Mechref, Pilsoo Kang, Milos V. Novotny, James P. Reilly

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Permethylated acidic and neutral N-glycans representing different types of glycan structures, such as linear and branched sialylated structures, high-mannose type and fucosylated complex type, were photodissociated with 157 nm vacuum ultraviolet light in a linear ion trap. Cross-ring fragments corresponding to high-energy fragmentation pathways were observed in abundance for all studied structures. Some product ions appear diagnostic for a linkage of sialic acid residues and the glycan antenna to which these residues are attached. A conclusive assignment of the fucosylation site of the studied glycan structure has been facilitated through measurement of cross-ring fragmentation resulting from photodissociation.

Original languageEnglish
Pages (from-to)1452-1460
Number of pages9
JournalRapid Communications in Mass Spectrometry
Volume21
Issue number8
DOIs
StatePublished - 2007

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