Isolation and expression of an Arabidopsis 14-3-3-like protein gene

Hong Zhang; Jing Wang; Inhwan Hwang; Howard M. Goodman

doi:10.1016/0167-4889(95)00006-E

Isolation and expression of an Arabidopsis 14-3-3-like protein gene

Hong Zhang, Jing Wang, Inhwan Hwang, Howard M. Goodman

Biological Sciences

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Abstract

We have isolated an Arabidopsis gene, AFT1, which encodes a 14-3-3-like protein (AFT1). The wide distribution of the 14-3-3 protein family in eukaryotes and the high steady-state mRNA levels of the AFT1 gene in most tissues throughout Arabidopsis growth and development suggest that AFT1 plays an important role(s) in Arabidopsis. DNA blot analysis indicates that AFT1 is a single copy gene, therefore AFT1 should be a good target for studies involving antisense or sense RNA technologies as a means to determine AFT1's function in vivo.

Original language	English
Pages (from-to)	113-116
Number of pages	4
Journal	BBA - Molecular Cell Research
Volume	1266
Issue number	1
DOIs	https://doi.org/10.1016/0167-4889(95)00006-E
State	Published - Apr 6 1995

Keywords

14-3-3 protein
Arabidopsis
Cell-signaling
Protein kinase C inhibitor

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10.1016/0167-4889(95)00006-E

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title = "Isolation and expression of an Arabidopsis 14-3-3-like protein gene",

abstract = "We have isolated an Arabidopsis gene, AFT1, which encodes a 14-3-3-like protein (AFT1). The wide distribution of the 14-3-3 protein family in eukaryotes and the high steady-state mRNA levels of the AFT1 gene in most tissues throughout Arabidopsis growth and development suggest that AFT1 plays an important role(s) in Arabidopsis. DNA blot analysis indicates that AFT1 is a single copy gene, therefore AFT1 should be a good target for studies involving antisense or sense RNA technologies as a means to determine AFT1's function in vivo.",

keywords = "14-3-3 protein, Arabidopsis, Cell-signaling, Protein kinase C inhibitor",

author = "Hong Zhang and Jing Wang and Inhwan Hwang and Goodman, {Howard M.}",

note = "Funding Information: We thank Deverie Bongard-Pierce, Gabor Lazor, and Jen Sheen for critically reading the manuscript, Lu Huang and Sun Lin for Arabidopsis Columbia and Landsberg DNAs, Russ Finley and Andrew Mendelsohn for advice throughout the interaction trap experiment, and Roger Brent's laboratory for materials used in the interaction trap experiment. This work was supported by a grant from Hoechst, A.G. (Frankfurt, Germany) to Massachusetts General Hospital.",

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doi = "10.1016/0167-4889(95)00006-E",

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AU - Zhang, Hong

AU - Wang, Jing

AU - Hwang, Inhwan

AU - Goodman, Howard M.

N1 - Funding Information: We thank Deverie Bongard-Pierce, Gabor Lazor, and Jen Sheen for critically reading the manuscript, Lu Huang and Sun Lin for Arabidopsis Columbia and Landsberg DNAs, Russ Finley and Andrew Mendelsohn for advice throughout the interaction trap experiment, and Roger Brent's laboratory for materials used in the interaction trap experiment. This work was supported by a grant from Hoechst, A.G. (Frankfurt, Germany) to Massachusetts General Hospital.

PY - 1995/4/6

Y1 - 1995/4/6

N2 - We have isolated an Arabidopsis gene, AFT1, which encodes a 14-3-3-like protein (AFT1). The wide distribution of the 14-3-3 protein family in eukaryotes and the high steady-state mRNA levels of the AFT1 gene in most tissues throughout Arabidopsis growth and development suggest that AFT1 plays an important role(s) in Arabidopsis. DNA blot analysis indicates that AFT1 is a single copy gene, therefore AFT1 should be a good target for studies involving antisense or sense RNA technologies as a means to determine AFT1's function in vivo.

AB - We have isolated an Arabidopsis gene, AFT1, which encodes a 14-3-3-like protein (AFT1). The wide distribution of the 14-3-3 protein family in eukaryotes and the high steady-state mRNA levels of the AFT1 gene in most tissues throughout Arabidopsis growth and development suggest that AFT1 plays an important role(s) in Arabidopsis. DNA blot analysis indicates that AFT1 is a single copy gene, therefore AFT1 should be a good target for studies involving antisense or sense RNA technologies as a means to determine AFT1's function in vivo.

KW - 14-3-3 protein

KW - Arabidopsis

KW - Cell-signaling

KW - Protein kinase C inhibitor

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JF - BBA - Molecular Cell Research

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Isolation and expression of an Arabidopsis 14-3-3-like protein gene

Abstract

Keywords

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