Isolation and characterization of the gene from Pseudomonas syringae pv. phaseolicola encoding the phaseolotoxin-insensitive ornithine carbamoyltransferase

Gilberto Mosqueda, Guido Van den Broeck, Odila Saucedo, Ana Maria Bailey, Ariel Alvarez-Morales, Luis Herrera-Estrella

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

The gene coding for the phaseolotoxin-insensitive ornithine carbamoyltransferase (OCTase) from Pseudomonas syringae pv. phaseolicola has been cloned and sequenced. The gene has a deduced coding capacity for a polypeptide with a calculated M, of 36520 daltons. Comparison of the amino acid sequence of the OCTase enzymes encoded by the P. aeruginosa argF and the Escherichia coli argI and argF genes with the deduced sequence of the newly identified gene shows that 79 amino acid residues are strictly conserved in all four polypeptides; among these 7 out of 9 residues are involved in enzyme function. Of three amino acid regions that have been implicated in substrate binding or catalysis, two are strictly conserved, and the third involved in carbamoylphosphate binding differs. This correlates well with published data showing that phaseolotoxin competes for the carbamoylphosphate binding site in the phaseolotoxin-sensitive OCTases. We propose that the gene be named argK.

Original languageEnglish
Pages (from-to)461-466
Number of pages6
JournalMGG Molecular & General Genetics
Volume222
Issue number2-3
DOIs
StatePublished - Jul 1990

Keywords

  • Nucleotide sequence
  • Ornithine carbamoyltransferase
  • Phaseolotoxin tolerance
  • Pseudomonas syringae pv. phaseolicola

Fingerprint Dive into the research topics of 'Isolation and characterization of the gene from Pseudomonas syringae pv. phaseolicola encoding the phaseolotoxin-insensitive ornithine carbamoyltransferase'. Together they form a unique fingerprint.

  • Cite this