Isolation and characterization of genes encoding chaperonin 60β from Arabidopsis thaliana

Eduardo Zabaleta; Araceli Oropeza; Beatriz Jiménez; Graciela Salerno; Martin Crespi; Luis Herrera-Estrella

doi:10.1016/0378-1119(92)90685-I

Isolation and characterization of genes encoding chaperonin 60β from Arabidopsis thaliana

Eduardo Zabaleta, Araceli Oropeza, Beatriz Jiménez, Graciela Salerno, Martin Crespi, Luis Herrera-Estrella

Plant & Soil Science

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Chaperonins (Cpn) are implicated in the folding and assembly of multimeric proteins in plastids and mitochondria of eukaryotes and in prokaryotes. Plastid Cpn is composed of two different polypeptides termed Cpn60α and Cpn60β. We have isolated cDNA and genomic clones encoding Cpn60β from Arabidopsis thaliana. The steady-state level of the cpn60β mRNAs is higher in etiolated leaves and sucrose-treated plants as compared to control leaves. The A. thaliana cpn60β gene family consists of at least three different coding units. It was confirmed that Cpnβ-encoding genes have a high level of conservation among plants.

Original language	English
Pages (from-to)	175-181
Number of pages	7
Journal	Gene
Volume	111
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(92)90685-I
State	Published - Feb 15 1992

Keywords

Recombinant DNA
exon
genomic library
intron
phage λ vector
promoter sequence
sugar induction
wheat

Access to Document

10.1016/0378-1119(92)90685-I

Cite this

@article{9792a04174b043709fd912eeb159e09f,

title = "Isolation and characterization of genes encoding chaperonin 60β from Arabidopsis thaliana",

abstract = "Chaperonins (Cpn) are implicated in the folding and assembly of multimeric proteins in plastids and mitochondria of eukaryotes and in prokaryotes. Plastid Cpn is composed of two different polypeptides termed Cpn60α and Cpn60β. We have isolated cDNA and genomic clones encoding Cpn60β from Arabidopsis thaliana. The steady-state level of the cpn60β mRNAs is higher in etiolated leaves and sucrose-treated plants as compared to control leaves. The A. thaliana cpn60β gene family consists of at least three different coding units. It was confirmed that Cpnβ-encoding genes have a high level of conservation among plants.",

keywords = "Recombinant DNA, exon, genomic library, intron, phage λ vector, promoter sequence, sugar induction, wheat",

author = "Eduardo Zabaleta and Araceli Oropeza and Beatriz Jim{\'e}nez and Graciela Salerno and Martin Crespi and Luis Herrera-Estrella",

note = "Funding Information: We would also like to thank Plinio Guzmfin and Alejandra Mandel for providing genomic and eDNA libraries. L. H.-E. is a member of the Sistema Nacional de Investigadores, Mexico, and is indebted to the Howard Hughes Biomedical Institute for a research grant. E.Z. is a doctoral fellow and G.S. is a Carreer Investigator of CONICET, Argentina.",

year = "1992",

month = feb,

day = "15",

doi = "10.1016/0378-1119(92)90685-I",

language = "English",

volume = "111",

pages = "175--181",

journal = "Gene",

issn = "0378-1119",

number = "2",

}

TY - JOUR

T1 - Isolation and characterization of genes encoding chaperonin 60β from Arabidopsis thaliana

AU - Zabaleta, Eduardo

AU - Oropeza, Araceli

AU - Jiménez, Beatriz

AU - Salerno, Graciela

AU - Crespi, Martin

AU - Herrera-Estrella, Luis

N1 - Funding Information: We would also like to thank Plinio Guzmfin and Alejandra Mandel for providing genomic and eDNA libraries. L. H.-E. is a member of the Sistema Nacional de Investigadores, Mexico, and is indebted to the Howard Hughes Biomedical Institute for a research grant. E.Z. is a doctoral fellow and G.S. is a Carreer Investigator of CONICET, Argentina.

PY - 1992/2/15

Y1 - 1992/2/15

N2 - Chaperonins (Cpn) are implicated in the folding and assembly of multimeric proteins in plastids and mitochondria of eukaryotes and in prokaryotes. Plastid Cpn is composed of two different polypeptides termed Cpn60α and Cpn60β. We have isolated cDNA and genomic clones encoding Cpn60β from Arabidopsis thaliana. The steady-state level of the cpn60β mRNAs is higher in etiolated leaves and sucrose-treated plants as compared to control leaves. The A. thaliana cpn60β gene family consists of at least three different coding units. It was confirmed that Cpnβ-encoding genes have a high level of conservation among plants.

AB - Chaperonins (Cpn) are implicated in the folding and assembly of multimeric proteins in plastids and mitochondria of eukaryotes and in prokaryotes. Plastid Cpn is composed of two different polypeptides termed Cpn60α and Cpn60β. We have isolated cDNA and genomic clones encoding Cpn60β from Arabidopsis thaliana. The steady-state level of the cpn60β mRNAs is higher in etiolated leaves and sucrose-treated plants as compared to control leaves. The A. thaliana cpn60β gene family consists of at least three different coding units. It was confirmed that Cpnβ-encoding genes have a high level of conservation among plants.

KW - Recombinant DNA

KW - exon

KW - genomic library

KW - intron

KW - phage λ vector

KW - promoter sequence

KW - sugar induction

KW - wheat

UR - http://www.scopus.com/inward/record.url?scp=0026508705&partnerID=8YFLogxK

U2 - 10.1016/0378-1119(92)90685-I

DO - 10.1016/0378-1119(92)90685-I

M3 - Article

C2 - 1347275

AN - SCOPUS:0026508705

SN - 0378-1119

VL - 111

SP - 175

EP - 181

JO - Gene

JF - Gene

IS - 2

ER -

Isolation and characterization of genes encoding chaperonin 60β from Arabidopsis thaliana

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this