TY - JOUR
T1 - Investigation of characteristics of urea and butyrylcholine chloride biosensors based on ion-selective field-effect transistors modified by the incorporation of heat-treated zeolite Beta crystals
AU - Soy, Esin
AU - Arkhypova, Valentyna
AU - Soldatkin, Oleksandr
AU - Shelyakina, Margarita
AU - Dzyadevych, Sergei
AU - Warzywoda, Juliusz
AU - Sacco, Albert
AU - Akata, Burcu
N1 - Funding Information:
This study was partly supported by the Scientific and Technical Research Council of Turkey (TÜBİTAK) and partly by a European Union project with project number PIRSES-GA-2008-230802 . The support provided by METU-Central Laboratory is greatly acknowledged.
PY - 2012/10/1
Y1 - 2012/10/1
N2 - Urea and butyrylcholine chloride (BuChCl) biosensors were prepared by adsorption of urease and butyrylcholinesterase (BuChE) on heat-treated zeolite Beta crystals, which were incorporated into membranes deposited on ion-selective field-effect transistor (ISFET) surfaces. The responses, stabilities, and use for inhibition analysis of these biosensors were investigated. Different heat treatment procedures changed the amount of Bronsted acid sites without affecting the size, morphology, overall Si/Al ratio, external specific surface area, and the amount of terminal silanol groups in zeolite crystals. Upon zeolite incorporation the enzymatic responses of biosensors towards urea and BuChCl increased up to ~ 2 and ~ 5 times, respectively; and correlated with the amount of Bronsted acid sites. All biosensors demonstrated high signal reproducibility and stability for both urease and BuChE. The inhibition characteristics of urease and BuChE were also related to the Bronsted acidity. The pore volume and pore size increases measured for the heat-treated samples are very unlikely causes for the improvements observed in biosensors' performance, because urease and BuChE are approximately one order of magnitude larger than the resulting zeolite Beta pores. Overall, these results suggest that the zeolites incorporated into the biologically active membrane with enhanced Bronsted acidity can improve the performance of ISFET-based biosensors.
AB - Urea and butyrylcholine chloride (BuChCl) biosensors were prepared by adsorption of urease and butyrylcholinesterase (BuChE) on heat-treated zeolite Beta crystals, which were incorporated into membranes deposited on ion-selective field-effect transistor (ISFET) surfaces. The responses, stabilities, and use for inhibition analysis of these biosensors were investigated. Different heat treatment procedures changed the amount of Bronsted acid sites without affecting the size, morphology, overall Si/Al ratio, external specific surface area, and the amount of terminal silanol groups in zeolite crystals. Upon zeolite incorporation the enzymatic responses of biosensors towards urea and BuChCl increased up to ~ 2 and ~ 5 times, respectively; and correlated with the amount of Bronsted acid sites. All biosensors demonstrated high signal reproducibility and stability for both urease and BuChE. The inhibition characteristics of urease and BuChE were also related to the Bronsted acidity. The pore volume and pore size increases measured for the heat-treated samples are very unlikely causes for the improvements observed in biosensors' performance, because urease and BuChE are approximately one order of magnitude larger than the resulting zeolite Beta pores. Overall, these results suggest that the zeolites incorporated into the biologically active membrane with enhanced Bronsted acidity can improve the performance of ISFET-based biosensors.
KW - Butyrylcholinesterase
KW - Ion sensitive field effect transistor
KW - Urea
KW - Urease
KW - Zeolite Beta
KW - Zeolite acidity
UR - http://www.scopus.com/inward/record.url?scp=84863322002&partnerID=8YFLogxK
U2 - 10.1016/j.msec.2012.04.071
DO - 10.1016/j.msec.2012.04.071
M3 - Article
AN - SCOPUS:84863322002
VL - 32
SP - 1835
EP - 1842
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
SN - 0928-4931
IS - 7
ER -