Investigation of a submerged membrane reactor for continuous biomass hydrolysis

Mohammadmahdi Malmali, Jonathan Stickel, S. Ranil Wickramasinghe

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


Enzymatic hydrolysis of cellulose is one of the most costly steps in the bioconversion of lignocellulosic biomass. Use of a submerged membrane reactor has been investigated for continuous enzymatic hydrolysis of cellulose thus allowing for greater use of the enzyme compared to a batch process. The submerged 0.65 μm polyethersulfone microfiltration membrane avoids the need to pump a cellulose slurry through an external loop. Permeate containing glucose is withdrawn at pressures slightly below atmospheric pressure. The membrane rejects cellulose particles and cellulase enzyme bound to cellulose. Here proof-of-concept experiments have been conducted using a modified, commercially available membrane filtration cell under low fluxes around 75 L/(m2 h). The operating flux is determined by the rate of glucose production. Maximizing the rate of glucose production involves optimizing mixing, reactor holding time, and the time the feed is held in the reactor prior to commencement of membrane filtration and continuous operation. Maximizing glucose production rates will require operating at low glucose concentration in order to minimize the adverse effects of product inhibition. Consequently practical submerged membrane systems will require a combined sugar concentration step in order to concentrate the product sugar stream prior to fermentation.

Original languageEnglish
Pages (from-to)189-197
Number of pages9
JournalFood and Bioproducts Processing
StatePublished - Aug 23 2015


  • Biorefinery
  • Cellulase enzyme
  • Cellulose
  • Microfiltration
  • Product inhibition
  • Ultrafiltration


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