Neck musculoskeletal disorders have been associated with various occupational tasks, in particular tasks that require non-neutral sustained exertions. To gain a clear understanding of the neck biomechanics during such exertions, we have recently initiated an unprecedented integration of multi-modality state-of-the-art measurement procedures including dynamic radiographic imaging, surface-based motion capture, electromyography, computed tomography and magnetic resonance imaging. This paper describes an overview of our systematic, integrative efforts of in vivo biodynamic measurements during sustained-till-exhaustion neck exertions and multi-modality imaging data, and how such an integrated database can be used to construct subject-specific neck musculoskeletal models. A complete dataset of one participant is presented to illustrate the acquired data. In the next phase, subject-specific 'what-if' computer simulations will be implemented to understand the mechano-physiological effects of sustained-till-exhaustion neck exertions for different work scenarios and worker characteristics in order to derive effective injury prevention and intervention strategies.