Insulin increases lipogenic enzyme activity in human adipocytes in primary culture

Naïma Moustaïd, Brynn H. Jones, James W. Taylor

Research output: Contribution to journalArticlepeer-review

75 Scopus citations


Studies with human adipose tissue have demonstrated the presence of key enzymes of fat synthesis. However, long-term regulation of these enzymes has not been reported. To address this issue, we used human adipocytes in primary culture. Human adipose tissue was obtained from abdominal fat of patients undergoing abdominal surgery. Adipocytes were isolated by collagenase digestion and cultured in media supplemented with 1% fetal bovine serum. To evaluate metabolic activity of cultured cells, we assessed the following during the culture: DNA pattern, cell size, glucose consumption and activities for two lipogenic enzymes, fatty acid synthase (FAS) and glycerol- 3-phosphate dehydrogenase (GPDH). Analysis of DNA pattern showed that human adipocytes cultured under the above conditions did not undergo cell apoptosis. In addition, no significant change in the cell size occurred during 22 d of culture. Glucose consumption by cultured cells was also constant during the culture and was 60% greater in the presence of 10 nmol/L of insulin. Treatment of cultured human adipocytes with insulin for 3-22 d increased GPDH and FAS activity by 60% and 2.8-fold, respectively, compared to cells cultured without insulin. Furthermore, the increase in FAS activity due to insulin treatment was dose dependent and maximal at 10 nmol/L. Our studies show for the first time that human adipocytes can be maintained viable and metabolically active for 2-3 wk in culture. Interestingly, cultured cells remain responsive to insulin. Therefore, this system will allow further characterization of long-term regulation of lipogenesis in human adipocytes and will be useful for developing pharmacological treatments of obesity.

Original languageEnglish
Pages (from-to)865-870
Number of pages6
JournalJournal of Nutrition
Issue number4
StatePublished - Apr 1996


  • fatty acid synthase
  • glycerol-3- phosphate dehydrogenase
  • human adipocytes
  • insulin
  • primary culture


Dive into the research topics of 'Insulin increases lipogenic enzyme activity in human adipocytes in primary culture'. Together they form a unique fingerprint.

Cite this