Increasing Platelet Concentration in Leukocyte-reduced Platelet Rich Plasma Decreases Collagen Gene Synthesis in Tendons

Stacey Boswell; Lauren Schnabel; Emily Sundman; T Minas

Increasing Platelet Concentration in Leukocyte-reduced Platelet Rich Plasma Decreases Collagen Gene Synthesis in Tendons

Stacey Boswell, Lauren Schnabel, Emily Sundman, T Minas

Veterinary Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Background: Platelet-rich plasma (PRP) is used for the treatment of tendinopathy. There are numerous PRP preparations, and the optimal combination of platelets and leukocytes is not known. Hypothesis: Within leukocyte-reduced PRP (lrPRP), there is a plateau effect of platelet concentration, with increasing platelet concentrations being detrimental to extracellular matrix synthesis. Study design: Controlled laboratory study. Methods: Different formulations of lrPRP with respect to the platelet:leukocyte ratio were generated from venous blood of 8 horses. Explants of the superficial digital flexor tendon were cultured in lrPRP products for 96 hours. Platelet-derived growth factor-BB (PDGF-BB), tumor necrosis factor-α (TNF-α), transforming growth factor-β1 (TGF-β

Original language	English
Pages (from-to)	42-49
Journal	American Journal of Sports Medicine
State	Published - Jan 2014

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title = "Increasing Platelet Concentration in Leukocyte-reduced Platelet Rich Plasma Decreases Collagen Gene Synthesis in Tendons",

abstract = "Background: Platelet-rich plasma (PRP) is used for the treatment of tendinopathy. There are numerous PRP preparations, and the optimal combination of platelets and leukocytes is not known. Hypothesis: Within leukocyte-reduced PRP (lrPRP), there is a plateau effect of platelet concentration, with increasing platelet concentrations being detrimental to extracellular matrix synthesis. Study design: Controlled laboratory study. Methods: Different formulations of lrPRP with respect to the platelet:leukocyte ratio were generated from venous blood of 8 horses. Explants of the superficial digital flexor tendon were cultured in lrPRP products for 96 hours. Platelet-derived growth factor-BB (PDGF-BB), tumor necrosis factor-α (TNF-α), transforming growth factor-β1 (TGF-β",

author = "Stacey Boswell and Lauren Schnabel and Emily Sundman and T Minas",

year = "2014",

month = jan,

language = "English",

pages = "42--49",

journal = "American Journal of Sports Medicine",

}

TY - JOUR

T1 - Increasing Platelet Concentration in Leukocyte-reduced Platelet Rich Plasma Decreases Collagen Gene Synthesis in Tendons

AU - Boswell, Stacey

AU - Schnabel, Lauren

AU - Sundman, Emily

AU - Minas, T

PY - 2014/1

Y1 - 2014/1

N2 - Background: Platelet-rich plasma (PRP) is used for the treatment of tendinopathy. There are numerous PRP preparations, and the optimal combination of platelets and leukocytes is not known. Hypothesis: Within leukocyte-reduced PRP (lrPRP), there is a plateau effect of platelet concentration, with increasing platelet concentrations being detrimental to extracellular matrix synthesis. Study design: Controlled laboratory study. Methods: Different formulations of lrPRP with respect to the platelet:leukocyte ratio were generated from venous blood of 8 horses. Explants of the superficial digital flexor tendon were cultured in lrPRP products for 96 hours. Platelet-derived growth factor-BB (PDGF-BB), tumor necrosis factor-α (TNF-α), transforming growth factor-β1 (TGF-β

AB - Background: Platelet-rich plasma (PRP) is used for the treatment of tendinopathy. There are numerous PRP preparations, and the optimal combination of platelets and leukocytes is not known. Hypothesis: Within leukocyte-reduced PRP (lrPRP), there is a plateau effect of platelet concentration, with increasing platelet concentrations being detrimental to extracellular matrix synthesis. Study design: Controlled laboratory study. Methods: Different formulations of lrPRP with respect to the platelet:leukocyte ratio were generated from venous blood of 8 horses. Explants of the superficial digital flexor tendon were cultured in lrPRP products for 96 hours. Platelet-derived growth factor-BB (PDGF-BB), tumor necrosis factor-α (TNF-α), transforming growth factor-β1 (TGF-β

M3 - Article

SP - 42

EP - 49

JO - American Journal of Sports Medicine

JF - American Journal of Sports Medicine

ER -