TY - JOUR
T1 - In vitro bioactive metabolite production and plant regeneration of medicinal plant Angelica sinensis
AU - Huang, Tianmiao
AU - Liu, Di
AU - Cui, Xiuwen
AU - Li, Meiling
AU - Jin, Ling
AU - Paré, Paul W.
AU - Li, Mengfei
AU - Wei, Jianhe
N1 - Publisher Copyright:
© 2023 Elsevier B.V.
PY - 2023/4
Y1 - 2023/4
N2 - The root of Angelica sinensis (Oliv.) Diels is a traditional Chinese medicine (TCM) widely used for the cardio-cerebrovascular, anti-inflammatory, and anti-cancer agents, etc. Currently, the root yield is mainly limited by narrow geographical distribution, early bolting and flowering (EBF), continuous cropping obstacle, and germplasm degeneration. While two ancillary strategies to produce bioactive metabolites by bioreactor and innovate new germplasms by genome editing have not been conducted. Here is reported embryogenic callus induction, histomorphological structure identification, suspension cell culture, bioactive metabolite determination, shoot bud and root differentiation, and plantlet acclimatization at different treatments such as explants, hormones, and culture conditions. The embryogenic calli can be effectively induced (99.0%) from the root of aseptic seedlings by a combination of 2.0 mg/L IBA and 0.2 mg/L KT, and embryogenic cells including: three-cell, globular-, heart-, torpedo-, and cotyledon-shaped embryos were observed. The maximum growth rate, bioactive metabolites contents (i.e., ferulic acid, soluble sugar, flavonoids, and phenolic), and antioxidant capacities were observed at 9–12 d with inoculum density 40 g/L, 22 ℃ and 16/8 h light/dark. Robust plantlets can be obtained within 180 d after successive shoot bud and root differentiation as well as plantlet acclimation. Based on the strategies for the satisfaction of root demand, an effective and complete scheme is proposed for producing bioactive metabolites and regenerating plant of A. sinensis.
AB - The root of Angelica sinensis (Oliv.) Diels is a traditional Chinese medicine (TCM) widely used for the cardio-cerebrovascular, anti-inflammatory, and anti-cancer agents, etc. Currently, the root yield is mainly limited by narrow geographical distribution, early bolting and flowering (EBF), continuous cropping obstacle, and germplasm degeneration. While two ancillary strategies to produce bioactive metabolites by bioreactor and innovate new germplasms by genome editing have not been conducted. Here is reported embryogenic callus induction, histomorphological structure identification, suspension cell culture, bioactive metabolite determination, shoot bud and root differentiation, and plantlet acclimatization at different treatments such as explants, hormones, and culture conditions. The embryogenic calli can be effectively induced (99.0%) from the root of aseptic seedlings by a combination of 2.0 mg/L IBA and 0.2 mg/L KT, and embryogenic cells including: three-cell, globular-, heart-, torpedo-, and cotyledon-shaped embryos were observed. The maximum growth rate, bioactive metabolites contents (i.e., ferulic acid, soluble sugar, flavonoids, and phenolic), and antioxidant capacities were observed at 9–12 d with inoculum density 40 g/L, 22 ℃ and 16/8 h light/dark. Robust plantlets can be obtained within 180 d after successive shoot bud and root differentiation as well as plantlet acclimation. Based on the strategies for the satisfaction of root demand, an effective and complete scheme is proposed for producing bioactive metabolites and regenerating plant of A. sinensis.
KW - Angelica sinensis
KW - Bioactive metabolite
KW - Culture condition
KW - Histomorphological structure
KW - Plant regeneration
KW - Suspension cell culture
UR - http://www.scopus.com/inward/record.url?scp=85146433528&partnerID=8YFLogxK
U2 - 10.1016/j.indcrop.2023.116276
DO - 10.1016/j.indcrop.2023.116276
M3 - Article
AN - SCOPUS:85146433528
SN - 0926-6690
VL - 194
JO - Industrial Crops and Products
JF - Industrial Crops and Products
M1 - 116276
ER -