In vitro bioactive metabolite production and plant regeneration of medicinal plant Angelica sinensis

Tianmiao Huang, Di Liu, Xiuwen Cui, Meiling Li, Ling Jin, Paul W. Paré, Mengfei Li, Jianhe Wei

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The root of Angelica sinensis (Oliv.) Diels is a traditional Chinese medicine (TCM) widely used for the cardio-cerebrovascular, anti-inflammatory, and anti-cancer agents, etc. Currently, the root yield is mainly limited by narrow geographical distribution, early bolting and flowering (EBF), continuous cropping obstacle, and germplasm degeneration. While two ancillary strategies to produce bioactive metabolites by bioreactor and innovate new germplasms by genome editing have not been conducted. Here is reported embryogenic callus induction, histomorphological structure identification, suspension cell culture, bioactive metabolite determination, shoot bud and root differentiation, and plantlet acclimatization at different treatments such as explants, hormones, and culture conditions. The embryogenic calli can be effectively induced (99.0%) from the root of aseptic seedlings by a combination of 2.0 mg/L IBA and 0.2 mg/L KT, and embryogenic cells including: three-cell, globular-, heart-, torpedo-, and cotyledon-shaped embryos were observed. The maximum growth rate, bioactive metabolites contents (i.e., ferulic acid, soluble sugar, flavonoids, and phenolic), and antioxidant capacities were observed at 9–12 d with inoculum density 40 g/L, 22 ℃ and 16/8 h light/dark. Robust plantlets can be obtained within 180 d after successive shoot bud and root differentiation as well as plantlet acclimation. Based on the strategies for the satisfaction of root demand, an effective and complete scheme is proposed for producing bioactive metabolites and regenerating plant of A. sinensis.

Original languageEnglish
Article number116276
JournalIndustrial Crops and Products
Volume194
DOIs
StatePublished - Apr 2023

Keywords

  • Angelica sinensis
  • Bioactive metabolite
  • Culture condition
  • Histomorphological structure
  • Plant regeneration
  • Suspension cell culture

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