TY - JOUR
T1 - In Vitro and In Vivo Investigations of Antimicrobial Treatments to Reduce Escherichia coli O157
T2 - H7 in Cattle Manure
AU - Lee, M. S.
AU - Krumpelman, S. L.
AU - Apple, J. K.
AU - Yancey, J. W.S.
AU - Kegley, E. B.
AU - Johnson, M. G.
AU - Brashears, M. M.
AU - Stephens, T. P.
N1 - Publisher Copyright:
© 2009 American Registry of Professional Animal Scientists.
PY - 2009/2/1
Y1 - 2009/2/1
N2 - In Exp. 1, acetic acid, aluminum sulfate, cetylpyridinium chloride (CPC), lactic acid (LAC), or granulated sulfuric acid was applied to autoclaved manure inoculated with Escherichia coli O157:H7 carrying plasmids encoding green-fluorescent protein (1 mL E. coli O157:H7 culture/50 g manure). Treatment with LAC and CPC reduced E. coli O157:H7 by 1.98 and 1.99 log10 cfu/g, respectively. In Exp. 2, LAC or CPC was applied to unautoclaved, inoculated manure incubated at either 5 or 37°C. Regardless of incubation temperature, CPC was most effective at reducing E. coli O157:H7, whereas LAC reduced E. coli O157:H7, but only 48 h after treatment application. In Exp. 3, 90 crossbred heifers were blocked by BW and assigned to 15 separate pens (6 heifers/pen). Within blocks, pens (5 pens/treatment) were allocated randomly to 1) untreated pens; 2) pens treated with 1% CPC; or 3) pens treated with 5% LAC. One day after pen treatment and cattle placement, E. coli O157:H7 was reduced by almost 2.0 log10 most probable number/m2 by treating pens with either CPC or LAC, but E. coli O157:H7 counts were similar 28, 50, and 55 d after initial sanitation (treatment × sampling time, P < 0.01). Interestingly, 14 and 28 d after pen placement, heifers in LAC-treated pens tended to have greater (P ≤ 0.10) ADG than heifers in CPC-treated pens. Thus, results of this series of experiments suggest that sanitizing drylot pens before cattle placement may reduce the potential of animal contamination with E. coli O157:H7.
AB - In Exp. 1, acetic acid, aluminum sulfate, cetylpyridinium chloride (CPC), lactic acid (LAC), or granulated sulfuric acid was applied to autoclaved manure inoculated with Escherichia coli O157:H7 carrying plasmids encoding green-fluorescent protein (1 mL E. coli O157:H7 culture/50 g manure). Treatment with LAC and CPC reduced E. coli O157:H7 by 1.98 and 1.99 log10 cfu/g, respectively. In Exp. 2, LAC or CPC was applied to unautoclaved, inoculated manure incubated at either 5 or 37°C. Regardless of incubation temperature, CPC was most effective at reducing E. coli O157:H7, whereas LAC reduced E. coli O157:H7, but only 48 h after treatment application. In Exp. 3, 90 crossbred heifers were blocked by BW and assigned to 15 separate pens (6 heifers/pen). Within blocks, pens (5 pens/treatment) were allocated randomly to 1) untreated pens; 2) pens treated with 1% CPC; or 3) pens treated with 5% LAC. One day after pen treatment and cattle placement, E. coli O157:H7 was reduced by almost 2.0 log10 most probable number/m2 by treating pens with either CPC or LAC, but E. coli O157:H7 counts were similar 28, 50, and 55 d after initial sanitation (treatment × sampling time, P < 0.01). Interestingly, 14 and 28 d after pen placement, heifers in LAC-treated pens tended to have greater (P ≤ 0.10) ADG than heifers in CPC-treated pens. Thus, results of this series of experiments suggest that sanitizing drylot pens before cattle placement may reduce the potential of animal contamination with E. coli O157:H7.
KW - Beef cattle
KW - Cetylpyridinium chloride
KW - Escherichia coli O157:H7
KW - Inoculated manure
KW - Lactic acid
KW - Pen sanitation
UR - http://www.scopus.com/inward/record.url?scp=84874957986&partnerID=8YFLogxK
U2 - 10.15232/S1080-7446(15)30680-X
DO - 10.15232/S1080-7446(15)30680-X
M3 - Article
AN - SCOPUS:84874957986
SN - 1080-7446
VL - 25
SP - 49
EP - 59
JO - Professional Animal Scientist
JF - Professional Animal Scientist
IS - 1
ER -