Improving transformation efficiency of Arabidopsis thaliana by modifying the floral dip method

Miguel Martinez-Trujillo, Veronica Limones-Briones, José Luis Cabrera-Ponce, Luis Herrera-Estrella

Research output: Contribution to journalArticle

96 Scopus citations

Abstract

Arabidopsis thaliana transformation with the floral dip method was improved by modifying the cell density and mode of application of the Agrobacterium inoculum. Drops of inoculum were applied 4 times to closed flower buds. The inoculum OD600 was increased from 0.8 to more than 2.0. These modifications improved reproducibility and increased transformation efficiencies to 2-3%.

Original languageEnglish
Pages (from-to)63-70
Number of pages8
JournalPlant Molecular Biology Reporter
Volume22
Issue number1
DOIs
StatePublished - Mar 2004

Keywords

  • Arabidopsis
  • Efficiency
  • Transformation

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