Improving transformation efficiency of Arabidopsis thaliana by modifying the floral dip method

Miguel Martinez-Trujillo; Veronica Limones-Briones; José Luis Cabrera-Ponce; Luis Herrera-Estrella

doi:10.1007/BF02773350

Improving transformation efficiency of Arabidopsis thaliana by modifying the floral dip method

Miguel Martinez-Trujillo, Veronica Limones-Briones, José Luis Cabrera-Ponce, Luis Herrera-Estrella

Plant & Soil Science

Research output: Contribution to journal › Article › peer-review

112 Scopus citations

Abstract

Arabidopsis thaliana transformation with the floral dip method was improved by modifying the cell density and mode of application of the Agrobacterium inoculum. Drops of inoculum were applied 4 times to closed flower buds. The inoculum OD600 was increased from 0.8 to more than 2.0. These modifications improved reproducibility and increased transformation efficiencies to 2-3%.

Original language	English
Pages (from-to)	63-70
Number of pages	8
Journal	Plant Molecular Biology Reporter
Volume	22
Issue number	1
DOIs	https://doi.org/10.1007/BF02773350
State	Published - Mar 2004

Keywords

Arabidopsis
Efficiency
Transformation

Access to Document

10.1007/BF02773350

Cite this

@article{e48cb36935c34493b29ac0af0489aa49,

title = "Improving transformation efficiency of Arabidopsis thaliana by modifying the floral dip method",

abstract = "Arabidopsis thaliana transformation with the floral dip method was improved by modifying the cell density and mode of application of the Agrobacterium inoculum. Drops of inoculum were applied 4 times to closed flower buds. The inoculum OD600 was increased from 0.8 to more than 2.0. These modifications improved reproducibility and increased transformation efficiencies to 2-3%.",

keywords = "Arabidopsis, Efficiency, Transformation",

author = "Miguel Martinez-Trujillo and Veronica Limones-Briones and Cabrera-Ponce, {Jos{\'e} Luis} and Luis Herrera-Estrella",

note = "Funding Information: This research was supported in part by grants from the HHMI (75191-526901) and the Rockefeller Foundation (AS 9644) to L.H.-E. We are grateful to Beatriz Jim6nez and Guillermo Corona for synthesis of the oligonucleotides.",

year = "2004",

month = mar,

doi = "10.1007/BF02773350",

language = "English",

volume = "22",

pages = "63--70",

journal = "Plant Molecular Biology Reporter",

issn = "0735-9640",

publisher = "SPRINGER LINK",

number = "1",

}

TY - JOUR

T1 - Improving transformation efficiency of Arabidopsis thaliana by modifying the floral dip method

AU - Martinez-Trujillo, Miguel

AU - Limones-Briones, Veronica

AU - Cabrera-Ponce, José Luis

AU - Herrera-Estrella, Luis

N1 - Funding Information: This research was supported in part by grants from the HHMI (75191-526901) and the Rockefeller Foundation (AS 9644) to L.H.-E. We are grateful to Beatriz Jim6nez and Guillermo Corona for synthesis of the oligonucleotides.

PY - 2004/3

Y1 - 2004/3

N2 - Arabidopsis thaliana transformation with the floral dip method was improved by modifying the cell density and mode of application of the Agrobacterium inoculum. Drops of inoculum were applied 4 times to closed flower buds. The inoculum OD600 was increased from 0.8 to more than 2.0. These modifications improved reproducibility and increased transformation efficiencies to 2-3%.

AB - Arabidopsis thaliana transformation with the floral dip method was improved by modifying the cell density and mode of application of the Agrobacterium inoculum. Drops of inoculum were applied 4 times to closed flower buds. The inoculum OD600 was increased from 0.8 to more than 2.0. These modifications improved reproducibility and increased transformation efficiencies to 2-3%.

KW - Arabidopsis

KW - Efficiency

KW - Transformation

UR - http://www.scopus.com/inward/record.url?scp=18944366388&partnerID=8YFLogxK

U2 - 10.1007/BF02773350

DO - 10.1007/BF02773350

M3 - Article

AN - SCOPUS:18944366388

SN - 0735-9640

VL - 22

SP - 63

EP - 70

JO - Plant Molecular Biology Reporter

JF - Plant Molecular Biology Reporter

IS - 1

ER -

Improving transformation efficiency of Arabidopsis thaliana by modifying the floral dip method

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this