Abstract
Arabidopsis thaliana transformation with the floral dip method was improved by modifying the cell density and mode of application of the Agrobacterium inoculum. Drops of inoculum were applied 4 times to closed flower buds. The inoculum OD600 was increased from 0.8 to more than 2.0. These modifications improved reproducibility and increased transformation efficiencies to 2-3%.
Original language | English |
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Pages (from-to) | 63-70 |
Number of pages | 8 |
Journal | Plant Molecular Biology Reporter |
Volume | 22 |
Issue number | 1 |
DOIs | |
State | Published - Mar 2004 |
Keywords
- Arabidopsis
- Efficiency
- Transformation