TY - JOUR
T1 - Improved collision-induced dissociation analysis of peptides by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry through 3-sulfobenzoic acid succinimidyl ester labeling
AU - Alley, William R.
AU - Mechref, Yehia
AU - Klouckova, Iveta
AU - Novotny, Milos V.
PY - 2007/1
Y1 - 2007/1
N2 - The sulfonation reagent, a succinimidyl ester of 3-sulfobenzoic acid, has been synthesized for effective peptide sequencing. It is capable of incorporating an additional mobile proton into the peptide backbone, thus, facilitating efficient collision-induced dissociation. This reagent is easily and inexpensively prepared in short time. Tandem mass spectra of the guanidinated and reagent-sulfonated peptides consist mainly of the y-ion series with higher intensities than those observed for solely guanidinated peptides. These enhanced tandem MS attributes significantly improved MASCOT total-ion scores, thus, allowing more confident peptide sequencing. This derivatization was also very effective for the analysis of tryptic digest of human blood serum proteins separated by two-dimensional gel electrophoresis. When used in LC-MALDI/MS/MS format, this type of derivatization does not adversely affect chromatographic efficiencies.
AB - The sulfonation reagent, a succinimidyl ester of 3-sulfobenzoic acid, has been synthesized for effective peptide sequencing. It is capable of incorporating an additional mobile proton into the peptide backbone, thus, facilitating efficient collision-induced dissociation. This reagent is easily and inexpensively prepared in short time. Tandem mass spectra of the guanidinated and reagent-sulfonated peptides consist mainly of the y-ion series with higher intensities than those observed for solely guanidinated peptides. These enhanced tandem MS attributes significantly improved MASCOT total-ion scores, thus, allowing more confident peptide sequencing. This derivatization was also very effective for the analysis of tryptic digest of human blood serum proteins separated by two-dimensional gel electrophoresis. When used in LC-MALDI/MS/MS format, this type of derivatization does not adversely affect chromatographic efficiencies.
KW - 3-sulfobenzoic acid succinimidyl ester
KW - LC-MALDI/TOF/TOF tandem MS
KW - MALDI/TOF/HOF tandem MS
KW - Peptide sulfonation
UR - http://www.scopus.com/inward/record.url?scp=33846571319&partnerID=8YFLogxK
U2 - 10.1021/pr0602747
DO - 10.1021/pr0602747
M3 - Article
C2 - 17203956
AN - SCOPUS:33846571319
VL - 6
SP - 124
EP - 132
JO - Journal of Proteome Research
JF - Journal of Proteome Research
SN - 1535-3893
IS - 1
ER -