Newer genetic lines of pigs are being used in indoor and outdoor production systems. The objectives of Exp. 1 were to describe the effects of the maternal sow line genotype, environment (indoor vs outdoor), and the genotype × environment interactions on blood hemoglobin (Hb), immunoglobulin G (IgG) concentrations, white blood cell (WBC) numbers, lymphocyte transformation/blastogenesis (LTA), natural killer (NK) cell activity, neutrophil chemotaxis, cortisol concentrations, and leukocyte differentials. Studies were performed using two genotypes: PIC Experimental-94 (Exp-94, an experimental line containing 25% Meishan) and PIC Camborough-15 (C-15). The Exp-94 sows had lower LTA at 0.2 μg/mL mitogen than the C-15 sows, whereas Exp-94 sows had higher NK cytotoxicity than the C-15 sows. When indoors, the two genotypes showed similar neutrophil chemotaxis. When outdoors, the C-15 genotype had higher (P < .01) neutrophil chemotaxis than the Exp-94 sows. The other immune measures were statistically similar for the two genotypes for each environment and for the genotype × environment interaction of sows. Experiment 2 sought to determine the effects of genotype on the immune system of nursery-age offspring of the experimental lines. Each sow line was bred to a common PIC 405 boar line. The Exp-94 × 405 pigs had elevated WBC numbers than C-15 × 405 pigs. The social status of the Exp-94 × 405 or the C-15 × 405 pigs showed no effect on any of the immune measures studied. The other immune measures were statistically similar for the two lines of pigs. The Exp-94 line had marginally increased NK activity but reduced lymphocyte blastogenesis and neutrophil chemotaxis compared with the C-15 line.