TY - JOUR
T1 - Identifying cancer biomarkers by mass spectrometry-based glycomics
AU - Mechref, Yehia
AU - Hu, Yunli
AU - Garcia, Aldo
AU - Hussein, Ahmed
PY - 2012/7
Y1 - 2012/7
N2 - Correlations between aberrant glycosylation and cancer have been established for decades. The major advances in mass spectrometry (MS) and separation science have rapidly advanced detailed characterization of the changes associated with cancer development and progression. Over the past 10 years, many reports have described MS-based glycomic methods directed toward comparing the glycomic profiles of different human specimens collected from disease-free individuals and patients with cancers. Glycomic profiling of glycoproteins isolated from human specimens originating from disease-free individuals and patients with cancers have also been performed. Profiling of native, labeled, and permethylated glycans has been acquired using MALDI-MS and LC-MS. This review focuses on describing, discussing, and evaluating the different glycomic methods employed to characterize and quantify glycomic changes associated with cancers of different organs, including breast, colon, esophagus, liver, ovarian, pancreas, and prostate.
AB - Correlations between aberrant glycosylation and cancer have been established for decades. The major advances in mass spectrometry (MS) and separation science have rapidly advanced detailed characterization of the changes associated with cancer development and progression. Over the past 10 years, many reports have described MS-based glycomic methods directed toward comparing the glycomic profiles of different human specimens collected from disease-free individuals and patients with cancers. Glycomic profiling of glycoproteins isolated from human specimens originating from disease-free individuals and patients with cancers have also been performed. Profiling of native, labeled, and permethylated glycans has been acquired using MALDI-MS and LC-MS. This review focuses on describing, discussing, and evaluating the different glycomic methods employed to characterize and quantify glycomic changes associated with cancers of different organs, including breast, colon, esophagus, liver, ovarian, pancreas, and prostate.
KW - Glycans
KW - Glycomics
KW - Glycoproteins
KW - LC-MS
KW - MALDI-MS
UR - http://www.scopus.com/inward/record.url?scp=84863006290&partnerID=8YFLogxK
U2 - 10.1002/elps.201100715
DO - 10.1002/elps.201100715
M3 - Review article
C2 - 22740464
AN - SCOPUS:84863006290
VL - 33
SP - 1755
EP - 1767
JO - Electrophoresis
JF - Electrophoresis
SN - 0173-0835
IS - 12
ER -