Identifying cancer biomarkers by mass spectrometry-based glycomics

Yehia Mechref, Yunli Hu, Aldo Garcia, Ahmed Hussein

Research output: Contribution to journalReview article

98 Scopus citations

Abstract

Correlations between aberrant glycosylation and cancer have been established for decades. The major advances in mass spectrometry (MS) and separation science have rapidly advanced detailed characterization of the changes associated with cancer development and progression. Over the past 10 years, many reports have described MS-based glycomic methods directed toward comparing the glycomic profiles of different human specimens collected from disease-free individuals and patients with cancers. Glycomic profiling of glycoproteins isolated from human specimens originating from disease-free individuals and patients with cancers have also been performed. Profiling of native, labeled, and permethylated glycans has been acquired using MALDI-MS and LC-MS. This review focuses on describing, discussing, and evaluating the different glycomic methods employed to characterize and quantify glycomic changes associated with cancers of different organs, including breast, colon, esophagus, liver, ovarian, pancreas, and prostate.

Original languageEnglish
Pages (from-to)1755-1767
Number of pages13
JournalELECTROPHORESIS
Volume33
Issue number12
DOIs
StatePublished - Jul 2012

Keywords

  • Glycans
  • Glycomics
  • Glycoproteins
  • LC-MS
  • MALDI-MS

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