TY - JOUR
T1 - Identification of Isomeric N-Glycan Structures by Mass Spectrometry with 157 nm Laser-Induced Photofragmentation
AU - Devakumar, Arugadoss
AU - Mechref, Yehia
AU - Kang, Pilsoo
AU - Novotny, Milos V.
AU - Reilly, James P.
N1 - Funding Information:
This work was supported by National Science Foundation Grants CHE-0518234 and CHE-0431991 and by a grant from the National Center for Research Resources, a component of the National Institutes of Health (NIH-NCRR) for the National Center for Glycomics and Glycoproteomics Grant RR018942.
PY - 2008/7
Y1 - 2008/7
N2 - Characterization of structural isomers has become increasingly important and extremely challenging in glycobiology. This communication demonstrates the capability of ion-trap mass spectrometry in conjunction with 157 nm photofragmentation to identify different structural isomers of permethylated N-glycans derived from ovalbumin without chromatographic separation. The results are compared with collision-induced dissociation (CID) experiments. Photodissociation generates extensive cross-ring fragment ions as well as diagnostic glycosidic product ions that are not usually observed in CID MS/MS experiments. The detection of these product ions aids in characterizing indigenous glycan isomers. The ion trap facilitates MSn experiments on the diagnostic glycosidic fragments and cross-ring product ions generated through photofragmentation, thus allowing unambiguous assignment of all of the isomeric structures associated with the model glycoprotein used in this study. Photofragmentation is demonstrated to be a powerful technique for the structural characterization of glycans.
AB - Characterization of structural isomers has become increasingly important and extremely challenging in glycobiology. This communication demonstrates the capability of ion-trap mass spectrometry in conjunction with 157 nm photofragmentation to identify different structural isomers of permethylated N-glycans derived from ovalbumin without chromatographic separation. The results are compared with collision-induced dissociation (CID) experiments. Photodissociation generates extensive cross-ring fragment ions as well as diagnostic glycosidic product ions that are not usually observed in CID MS/MS experiments. The detection of these product ions aids in characterizing indigenous glycan isomers. The ion trap facilitates MSn experiments on the diagnostic glycosidic fragments and cross-ring product ions generated through photofragmentation, thus allowing unambiguous assignment of all of the isomeric structures associated with the model glycoprotein used in this study. Photofragmentation is demonstrated to be a powerful technique for the structural characterization of glycans.
UR - http://www.scopus.com/inward/record.url?scp=45849127234&partnerID=8YFLogxK
U2 - 10.1016/j.jasms.2008.03.005
DO - 10.1016/j.jasms.2008.03.005
M3 - Article
C2 - 18487060
AN - SCOPUS:45849127234
VL - 19
SP - 1027
EP - 1040
JO - Journal of the American Society for Mass Spectrometry
JF - Journal of the American Society for Mass Spectrometry
SN - 1044-0305
IS - 7
ER -