Identification of candidate network hubs involved in metabolic adjustments of rice under drought stress by integrating transcriptome data and genome-scale metabolic network

Bijayalaxmi Mohanty, Ai Kitazumi, C. Y.Maurice Cheung, Meiyappan Lakshmanan, Benildo G. de los Reyes, In Cheol Jang, Dong Yup Lee

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

In this study, we have integrated a rice genome-scale metabolic network and the transcriptome of a drought-tolerant rice line, DK151, to identify the major transcriptional regulators involved in metabolic adjustments necessary for adaptation to drought. This was achieved by examining the differential expressions of transcription factors and metabolic genes in leaf, root and young panicle of rice plants subjected to drought stress during tillering, booting and panicle elongation stages. Critical transcription factors such as AP2/ERF, bZIP, MYB and NAC that control the important nodes in the gene regulatory pathway were identified through correlative analysis of the patterns of spatio-temporal expression and cis-element enrichment. We showed that many of the candidate transcription factors involved in metabolic adjustments were previously linked to phenotypic variation for drought tolerance. This approach represents the first attempt to integrate models of transcriptional regulation and metabolic pathways for the identification of candidate regulatory genes for targeted selection in rice breeding.

Original languageEnglish
Pages (from-to)224-239
Number of pages16
JournalPlant Science
Volume242
DOIs
StatePublished - Jan 1 2016

Keywords

  • Cis-elements
  • Drought
  • Metabolic network
  • Physiological adjustment
  • Plant breeding
  • Transcription factor

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