Identification of bacteria causing acute otitis media using Raman microspectroscopy

Oscar D. Ayala, Catherine A. Wakeman, Eric P. Skaar, Anita Mahadevan-Jansen

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations


Otitis media (OM) is the leading cause of acute physician visits and prescription of antibiotics for children. Current standard techniques to diagnose acute otitis media (AOM) are limited by their ability to probe only changes in symptoms of the bacterial infection that cause AOM. Furthermore, they are not able to detect the presence of or identify bacteria causing AOM, which is important for diagnosis and proper antibiotic treatment. Our goal is to detect the presence of and identify the pathogens involved in causing AOM based on their biochemical profile using Raman spectroscopy (RS). An inVia confocal Raman microscope (Renishaw) at 785 nm was used to detect bacteria causing AOM in vitro. The three main bacteria that cause AOM, Haemophilus influenzae, Moraxella catarrhalis, and Streptococcus pneumoniae were cultured in chocolate agar and Mueller-Hinton agar to determine which agar type would minimize Raman signal from the growth agar. Preliminary results identified specific Raman spectral features characteristic of S. pneumoniae. RS has the potential to accurately diagnose AOM, which will help in identifying the antibiotic that will be most beneficial for the patient and ultimately decrease the course of infection.

Original languageEnglish
Title of host publicationBiomedical Vibrational Spectroscopy 2016
Subtitle of host publicationAdvances in Research and Industry
EditorsAnita Mahadevan-Jansen, Wolfgang Petrich
ISBN (Electronic)9781628419382
StatePublished - 2016
EventBiomedical Vibrational Spectroscopy 2016: Advances in Research and Industry - San Francisco, United States
Duration: Feb 13 2016Feb 14 2016

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceBiomedical Vibrational Spectroscopy 2016: Advances in Research and Industry
Country/TerritoryUnited States
CitySan Francisco


  • Raman microspectroscopy
  • Raman spectroscopy
  • acute otitis media (AOM)
  • agar
  • bacteria


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