TY - GEN
T1 - Identification of bacteria causing acute otitis media using Raman microspectroscopy
AU - Ayala, Oscar D.
AU - Wakeman, Catherine A.
AU - Skaar, Eric P.
AU - Mahadevan-Jansen, Anita
N1 - Publisher Copyright:
© 2016 SPIE.
Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 2016
Y1 - 2016
N2 - Otitis media (OM) is the leading cause of acute physician visits and prescription of antibiotics for children. Current standard techniques to diagnose acute otitis media (AOM) are limited by their ability to probe only changes in symptoms of the bacterial infection that cause AOM. Furthermore, they are not able to detect the presence of or identify bacteria causing AOM, which is important for diagnosis and proper antibiotic treatment. Our goal is to detect the presence of and identify the pathogens involved in causing AOM based on their biochemical profile using Raman spectroscopy (RS). An inVia confocal Raman microscope (Renishaw) at 785 nm was used to detect bacteria causing AOM in vitro. The three main bacteria that cause AOM, Haemophilus influenzae, Moraxella catarrhalis, and Streptococcus pneumoniae were cultured in chocolate agar and Mueller-Hinton agar to determine which agar type would minimize Raman signal from the growth agar. Preliminary results identified specific Raman spectral features characteristic of S. pneumoniae. RS has the potential to accurately diagnose AOM, which will help in identifying the antibiotic that will be most beneficial for the patient and ultimately decrease the course of infection.
AB - Otitis media (OM) is the leading cause of acute physician visits and prescription of antibiotics for children. Current standard techniques to diagnose acute otitis media (AOM) are limited by their ability to probe only changes in symptoms of the bacterial infection that cause AOM. Furthermore, they are not able to detect the presence of or identify bacteria causing AOM, which is important for diagnosis and proper antibiotic treatment. Our goal is to detect the presence of and identify the pathogens involved in causing AOM based on their biochemical profile using Raman spectroscopy (RS). An inVia confocal Raman microscope (Renishaw) at 785 nm was used to detect bacteria causing AOM in vitro. The three main bacteria that cause AOM, Haemophilus influenzae, Moraxella catarrhalis, and Streptococcus pneumoniae were cultured in chocolate agar and Mueller-Hinton agar to determine which agar type would minimize Raman signal from the growth agar. Preliminary results identified specific Raman spectral features characteristic of S. pneumoniae. RS has the potential to accurately diagnose AOM, which will help in identifying the antibiotic that will be most beneficial for the patient and ultimately decrease the course of infection.
KW - Raman microspectroscopy
KW - Raman spectroscopy
KW - acute otitis media (AOM)
KW - agar
KW - bacteria
UR - http://www.scopus.com/inward/record.url?scp=84973340572&partnerID=8YFLogxK
U2 - 10.1117/12.2213563
DO - 10.1117/12.2213563
M3 - Conference contribution
AN - SCOPUS:84973340572
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Biomedical Vibrational Spectroscopy 2016
A2 - Mahadevan-Jansen, Anita
A2 - Petrich, Wolfgang
PB - SPIE
Y2 - 13 February 2016 through 14 February 2016
ER -