Identification of Anopheles (Nyssorhynchus) marajoara (Diptera: Culicidae) in Bolivia using polymerase chain reaction and a restriction endonuclease

C. L. Brelsfoard; G. N. Fritz; R. Rodriguez

doi:10.1603/0013-8746(2006)99[707:IOANMD]2.0.CO;2

Identification of Anopheles (Nyssorhynchus) marajoara (Diptera: Culicidae) in Bolivia using polymerase chain reaction and a restriction endonuclease

C. L. Brelsfoard, G. N. Fritz, R. Rodriguez

Biological Sciences

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Anopheles (Nyssorhynchus) marajoara Galvão & Damasceno (Diptera: Culicidae) is a member of the Albitarsis cryptic species complex and is a primary vector of human Plasmodium in parts of Brazil. We report the first record of An. marajoara in the department of Cochabamba and confirm its presence in the department of Santa Cruz, Bolivia. We also describe a PCR protocol producing a single amplicon (≈340 bp) of a section of the internal transcribed spacer 2 specific for members of the Albitarsis species complex; the polymerase chain reaction protocol did not amplify the DNA of 17 other species in the subgenus Nyssorhynchus. Digestion of the amplicon with the restriction endonuclease BfaI produces two fragments specific to An. marajoara in Bolivia.

Original language	English
Pages (from-to)	707-713
Number of pages	7
Journal	Annals of the Entomological Society of America
Volume	99
Issue number	4
DOIs	https://doi.org/10.1603/0013-8746(2006)99[707:IOANMD]2.0.CO;2
State	Published - 2006

Keywords

Albitarsis Complex
Anopheles marajoara
Distribution
ITS2
Taxonomy

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10.1603/0013-8746(2006)99[707:IOANMD]2.0.CO;2

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title = "Identification of Anopheles (Nyssorhynchus) marajoara (Diptera: Culicidae) in Bolivia using polymerase chain reaction and a restriction endonuclease",

abstract = "Anopheles (Nyssorhynchus) marajoara Galv{\~a}o & Damasceno (Diptera: Culicidae) is a member of the Albitarsis cryptic species complex and is a primary vector of human Plasmodium in parts of Brazil. We report the first record of An. marajoara in the department of Cochabamba and confirm its presence in the department of Santa Cruz, Bolivia. We also describe a PCR protocol producing a single amplicon (≈340 bp) of a section of the internal transcribed spacer 2 specific for members of the Albitarsis species complex; the polymerase chain reaction protocol did not amplify the DNA of 17 other species in the subgenus Nyssorhynchus. Digestion of the amplicon with the restriction endonuclease BfaI produces two fragments specific to An. marajoara in Bolivia.",

keywords = "Albitarsis Complex, Anopheles marajoara, Distribution, ITS2, Taxonomy",

author = "Brelsfoard, {C. L.} and Fritz, {G. N.} and R. Rodriguez",

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AU - Fritz, G. N.

AU - Rodriguez, R.

PY - 2006

Y1 - 2006

N2 - Anopheles (Nyssorhynchus) marajoara Galvão & Damasceno (Diptera: Culicidae) is a member of the Albitarsis cryptic species complex and is a primary vector of human Plasmodium in parts of Brazil. We report the first record of An. marajoara in the department of Cochabamba and confirm its presence in the department of Santa Cruz, Bolivia. We also describe a PCR protocol producing a single amplicon (≈340 bp) of a section of the internal transcribed spacer 2 specific for members of the Albitarsis species complex; the polymerase chain reaction protocol did not amplify the DNA of 17 other species in the subgenus Nyssorhynchus. Digestion of the amplicon with the restriction endonuclease BfaI produces two fragments specific to An. marajoara in Bolivia.

AB - Anopheles (Nyssorhynchus) marajoara Galvão & Damasceno (Diptera: Culicidae) is a member of the Albitarsis cryptic species complex and is a primary vector of human Plasmodium in parts of Brazil. We report the first record of An. marajoara in the department of Cochabamba and confirm its presence in the department of Santa Cruz, Bolivia. We also describe a PCR protocol producing a single amplicon (≈340 bp) of a section of the internal transcribed spacer 2 specific for members of the Albitarsis species complex; the polymerase chain reaction protocol did not amplify the DNA of 17 other species in the subgenus Nyssorhynchus. Digestion of the amplicon with the restriction endonuclease BfaI produces two fragments specific to An. marajoara in Bolivia.

KW - Albitarsis Complex

KW - Anopheles marajoara

KW - Distribution

KW - ITS2

KW - Taxonomy

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ER -

Identification of Anopheles (Nyssorhynchus) marajoara (Diptera: Culicidae) in Bolivia using polymerase chain reaction and a restriction endonuclease

Abstract

Keywords

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