TY - JOUR
T1 - High specificity of a rare terrestrial orchid toward a rare fungus within the North American tallgrass prairie
AU - Kaur, Jaspreet
AU - Andrews, Lela
AU - Sharma, Jyotsna
N1 - Funding Information:
We gratefully acknowledge The U.S. Fish and Wildlife Service (USFWS; Grant# F12AP00638) and The State of Minnesota (Department of Natural Resources Grant# 3000038088 47630-10) for funding this project. We recognize the assistance in obtaining permits and technical assistance in the field from: Phil Delphey (USFWS), Nancy Sather (Minnesota Department of Natural Resources), Matthew Mecklenburg and Brian Winter (The Nature Conservancy Northern Tallgrass Prairie Ecoregion), Melvin Nenneman (Valentine National Wildlife Refuge, USFWS), Bryan Stotts (Dakota Prairie Grasslands, Sheyenne Ranger District), John Pearson (Iowa Department of Natural Resources), and Christie Borkowsky (Manitoba Tall Grass Prairie Preserve). We also thank Nancy Sather, Jeanne Prekker (volunteer), Donna Spaeth (volunteer), Derek Anderson (MNDNR), and Dan Svedarsky (University of Minnesota, Crookston) for assisting in the field or otherwise facilitating this research. Finally, we thank Mr. Pablo Tovar for generating fungal sequences from orchid roots for his master's degree thesis.
Funding Information:
We gratefully acknowledge The U.S. Fish and Wildlife Service (USFWS ; Grant# F12AP00638 ) and The State of Minnesota (Department of Natural Resources Grant# 3000038088 47630-10 ) for funding this project. We recognize the assistance in obtaining permits and technical assistance in the field from: Phil Delphey (USFWS), Nancy Sather (Minnesota Department of Natural Resources), Matthew Mecklenburg and Brian Winter (The Nature Conservancy Northern Tallgrass Prairie Ecoregion), Melvin Nenneman (Valentine National Wildlife Refuge, USFWS), Bryan Stotts (Dakota Prairie Grasslands, Sheyenne Ranger District), John Pearson (Iowa Department of Natural Resources), and Christie Borkowsky (Manitoba Tall Grass Prairie Preserve). We also thank Nancy Sather, Jeanne Prekker (volunteer), Donna Spaeth (volunteer), Derek Anderson (MNDNR), and Dan Svedarsky (University of Minnesota, Crookston) for assisting in the field or otherwise facilitating this research. Finally, we thank Mr. Pablo Tovar for generating fungal sequences from orchid roots for his master’s degree thesis.
Publisher Copyright:
© 2019 British Mycological Society
PY - 2019/12
Y1 - 2019/12
N2 - The Orchidaceae are globally distributed and represent a diverse lineage of obligate mycotrophic plants. Given their dependence on symbiotic fungi for germination and/or plant development, fungal community structure in substrates is expected to influence the distribution and persistence of orchid species. Yet, simultaneous characterization of orchid mycorrhizal fungal (OMF) communities in roots and in soil is rarely reported. To explain the co-distributions of OMF in roots, orchid-occupied, and bulk soil, we characterized mycorrhizal fungi associated with Platanthera praeclara over multiple years across its entire natural distribution within the North American tallgrass prairie. Root derived OMF communities included 24 Ceratobasidiaceae and 7 Tulasnellaceae operational taxonomic units (OTUs) though the orchid exhibited high spatio-temporal specificity toward a single Ceratobasidiaceae OTU, which was strongly stable across population sizes and phenological stages of the sampled individuals. The preferred OMF OTUs were primarily restricted to orchid-occupied locations while infrequent or absent in bulk soil. Variation in soil OMF assemblies was explained most by soil moisture, magnesium, manganese, and clay. In this first study of coupled root and soil OMF communities across a threatened grassland ecosystem, we report a strong relationship, further nuanced by soil chemistry, between a rare fungus and a rare orchid.
AB - The Orchidaceae are globally distributed and represent a diverse lineage of obligate mycotrophic plants. Given their dependence on symbiotic fungi for germination and/or plant development, fungal community structure in substrates is expected to influence the distribution and persistence of orchid species. Yet, simultaneous characterization of orchid mycorrhizal fungal (OMF) communities in roots and in soil is rarely reported. To explain the co-distributions of OMF in roots, orchid-occupied, and bulk soil, we characterized mycorrhizal fungi associated with Platanthera praeclara over multiple years across its entire natural distribution within the North American tallgrass prairie. Root derived OMF communities included 24 Ceratobasidiaceae and 7 Tulasnellaceae operational taxonomic units (OTUs) though the orchid exhibited high spatio-temporal specificity toward a single Ceratobasidiaceae OTU, which was strongly stable across population sizes and phenological stages of the sampled individuals. The preferred OMF OTUs were primarily restricted to orchid-occupied locations while infrequent or absent in bulk soil. Variation in soil OMF assemblies was explained most by soil moisture, magnesium, manganese, and clay. In this first study of coupled root and soil OMF communities across a threatened grassland ecosystem, we report a strong relationship, further nuanced by soil chemistry, between a rare fungus and a rare orchid.
KW - Mycorrhizae
KW - OMF
KW - Platanthera praeclara
KW - Root
KW - Soil
KW - Vegetation Management
UR - http://www.scopus.com/inward/record.url?scp=85073980056&partnerID=8YFLogxK
U2 - 10.1016/j.funbio.2019.09.010
DO - 10.1016/j.funbio.2019.09.010
M3 - Article
C2 - 31733732
AN - SCOPUS:85073980056
VL - 123
SP - 895
EP - 904
JO - Fungal Biology
JF - Fungal Biology
SN - 1878-6146
IS - 12
ER -