TY - JOUR
T1 - HaASR1 gene cloned from a desert shrub, Haloxylon ammodendron, confers drought tolerance in transgenic Arabidopsis thaliana
AU - Gao, Huijuan
AU - Lü, Xinpei
AU - Ren, Wei
AU - Sun, Yunya
AU - Zhao, Qi
AU - Wang, Guangpeng
AU - Wang, Runjuan
AU - Wang, Yongping
AU - Zhang, Hong
AU - Wang, Suomin
AU - Meng, Laisheng
AU - Zhang, Jinlin
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (grant No. 31222053 ) and Collaborative and Innovative Science and Technology Team Supporting Program for University in Gansu Province, China ( 2016C-05 ).
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/12
Y1 - 2020/12
N2 - Abiotic stresses pose adverse impact on plant growth and development and exploring the stress tolerance genes from dominant species is still urgent. The desert shrub, Haloxylon ammodendron, a C4 and succulent xero-halophyte species, has great drought and salt tolerance. Here, we reported the isolation and functions analysis of HaASR1 (abscisic acid, stress, and ripening) from H. ammodendron. HaASR1 interacts with HaPrxQ and HaBADH and may function in preventing PrxQ and BADH from denaturation in plants under drought stress. Overexpression of HaASR1 reduces the sensitivity of Arabidopsis plants to exogenous ABA and decreases the endogenous ABA level through down-regulating ABA biosynthesis genes under drought stress, which making the plants display less inhibition of shoot growth and enhanced drought tolerance. Overexpression of HaASR1 significantly enhances photosynthetic capacity of Arabidopsis plants under drought stress by reducing H2O2 accumulation through up-regulating AtCAT2, AtAPX1 and AtPrxQ and maintaining relatively integrity of chloroplasts. Overexpression of HaASR1 also significantly improves water-retaining ability. Taken together, we propose that HaASR1 plays critical roles in plant drought stress adaptation by making the plants partially defective in ABA signaling and participating in drought tolerance regulation through nonstomatal pathways, enhancing ROS-scavenging ability and water-retaining ability, which is different from the functions of previously reported ASR genes from other plant species. This research also reveals that HaASR1 gene from the desert shrub, Haloxylon ammodendron, has potential application values in improving abiotic stresses of other plant species by genetic engineering.
AB - Abiotic stresses pose adverse impact on plant growth and development and exploring the stress tolerance genes from dominant species is still urgent. The desert shrub, Haloxylon ammodendron, a C4 and succulent xero-halophyte species, has great drought and salt tolerance. Here, we reported the isolation and functions analysis of HaASR1 (abscisic acid, stress, and ripening) from H. ammodendron. HaASR1 interacts with HaPrxQ and HaBADH and may function in preventing PrxQ and BADH from denaturation in plants under drought stress. Overexpression of HaASR1 reduces the sensitivity of Arabidopsis plants to exogenous ABA and decreases the endogenous ABA level through down-regulating ABA biosynthesis genes under drought stress, which making the plants display less inhibition of shoot growth and enhanced drought tolerance. Overexpression of HaASR1 significantly enhances photosynthetic capacity of Arabidopsis plants under drought stress by reducing H2O2 accumulation through up-regulating AtCAT2, AtAPX1 and AtPrxQ and maintaining relatively integrity of chloroplasts. Overexpression of HaASR1 also significantly improves water-retaining ability. Taken together, we propose that HaASR1 plays critical roles in plant drought stress adaptation by making the plants partially defective in ABA signaling and participating in drought tolerance regulation through nonstomatal pathways, enhancing ROS-scavenging ability and water-retaining ability, which is different from the functions of previously reported ASR genes from other plant species. This research also reveals that HaASR1 gene from the desert shrub, Haloxylon ammodendron, has potential application values in improving abiotic stresses of other plant species by genetic engineering.
KW - ABA
KW - Drought tolerance
KW - HO
KW - HaASR1
KW - Haloxylon ammodendron
KW - Photosynthesis
UR - http://www.scopus.com/inward/record.url?scp=85090573438&partnerID=8YFLogxK
U2 - 10.1016/j.envexpbot.2020.104251
DO - 10.1016/j.envexpbot.2020.104251
M3 - Article
AN - SCOPUS:85090573438
VL - 180
JO - Environmental and Experimental Botany
JF - Environmental and Experimental Botany
SN - 0098-8472
M1 - 104251
ER -