TY - JOUR
T1 - Guided cell migration on a graded micropillar substrate
AU - Krishnamoorthy, Srikumar
AU - Zhang, Zhengyi
AU - Xu, Changxue
N1 - Publisher Copyright:
© 2020, Zhejiang University Press.
PY - 2020/3/1
Y1 - 2020/3/1
N2 - Cell migration is facilitated by the interaction of living cells and their local microenvironment. The local topography is one of the key factors regulating cell migration. Interaction between the surface topography and the cell behaviors is critical to understanding tissue development and regeneration. In this study, a dynamic mask photolithography technique has been utilized to fabricate a surface with graded micropillars. It has been demonstrated that the cells have been successfully guided to migrate from the sparse zone to the dense zone. The cell polarization angle has been characterized in both sparse zone and the dense zone. Compared to the dense zone, the cells in the sparse zone are more aligned along the direction of the micropillar spacing gradient, which enables the guided cell migration. Moreover, the effects of the micropillar spacing gradient, micropillar diameter, and micropillar height have been investigated in terms of the cell migration speed and cell spreading area. Finally, two issues significantly affecting the cell migration have been discussed: trapped cells between the micropillars and cell clusters.
AB - Cell migration is facilitated by the interaction of living cells and their local microenvironment. The local topography is one of the key factors regulating cell migration. Interaction between the surface topography and the cell behaviors is critical to understanding tissue development and regeneration. In this study, a dynamic mask photolithography technique has been utilized to fabricate a surface with graded micropillars. It has been demonstrated that the cells have been successfully guided to migrate from the sparse zone to the dense zone. The cell polarization angle has been characterized in both sparse zone and the dense zone. Compared to the dense zone, the cells in the sparse zone are more aligned along the direction of the micropillar spacing gradient, which enables the guided cell migration. Moreover, the effects of the micropillar spacing gradient, micropillar diameter, and micropillar height have been investigated in terms of the cell migration speed and cell spreading area. Finally, two issues significantly affecting the cell migration have been discussed: trapped cells between the micropillars and cell clusters.
KW - Cell polarization
KW - Graded microtopography
KW - Guided cell migration
UR - http://www.scopus.com/inward/record.url?scp=85078163441&partnerID=8YFLogxK
U2 - 10.1007/s42242-020-00059-7
DO - 10.1007/s42242-020-00059-7
M3 - Article
AN - SCOPUS:85078163441
SN - 2096-5524
VL - 3
SP - 60
EP - 70
JO - Bio-Design and Manufacturing
JF - Bio-Design and Manufacturing
IS - 1
ER -